Transcriptomic Analysis of Single Isolated Myofibers Identifies miR-27a-3p and miR-142-3p as Regulators of Metabolism in Skeletal Muscle.
lipids
miRNAs
mitochondria
single myofiber
skeletal muscle metabolism
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
26 03 2019
26 03 2019
Historique:
received:
05
03
2018
revised:
29
06
2018
accepted:
26
02
2019
entrez:
28
3
2019
pubmed:
28
3
2019
medline:
7
5
2020
Statut:
ppublish
Résumé
Skeletal muscle is composed of different myofiber types that preferentially use glucose or lipids for ATP production. How fuel preference is regulated in these post-mitotic cells is largely unknown, making this issue a key question in the fields of muscle and whole-body metabolism. Here, we show that microRNAs (miRNAs) play a role in defining myofiber metabolic profiles. mRNA and miRNA signatures of all myofiber types obtained at the single-cell level unveiled fiber-specific regulatory networks and identified two master miRNAs that coordinately control myofiber fuel preference and mitochondrial morphology. Our work provides a complete and integrated mouse myofiber type-specific catalog of gene and miRNA expression and establishes miR-27a-3p and miR-142-3p as regulators of lipid use in skeletal muscle.
Identifiants
pubmed: 30917329
pii: S2211-1247(19)30291-8
doi: 10.1016/j.celrep.2019.02.105
pii:
doi:
Substances chimiques
MIRN142 microRNA, human
0
MIRN27 microRNA, human
0
MicroRNAs
0
Glycogen
9005-79-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3784-3797.e8Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.