Monitoring β-Arrestin 2 Targeting to the Centrosome, Basal Body, and Primary Cilium by Fluorescence Microscopy.
Animals
Basal Bodies
/ drug effects
Cell Cycle
/ drug effects
Cell Line
Centrosome
/ drug effects
Cilia
/ drug effects
DNA
/ metabolism
Fluorescence Recovery After Photobleaching
Green Fluorescent Proteins
/ metabolism
Hippocampus
/ cytology
Humans
Mice
Microscopy, Fluorescence
/ methods
Microtubules
/ drug effects
Neurons
/ drug effects
Plasmids
/ metabolism
Somatostatin
/ pharmacology
beta-Arrestin 2
/ metabolism
Arrestin
Basal body
Cellular signaling
Centrosome
Primary cilia
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
29
3
2019
pubmed:
29
3
2019
medline:
19
7
2019
Statut:
ppublish
Résumé
Primary cilia (PC) are microtubule-based organelles that behave like a cellular antenna controlling key signaling pathways during development and tissue homeostasis. The ciliary membrane is highly enriched for G protein-coupled receptors (GPCRs), and PC are a crucial signaling compartment for this large receptor family. Downstream effectors of GPCR signaling are also present in cilia, and evidence obtained by our labs and others demonstrated that β-arrestin (βarr) family members are differentially recruited to PC and have investigated the role of GPCR activation in this process. In this chapter, we provide methods based on fluorescence microscopy on fixed or live cells suitable for investigating targeting and recruitment of βarrs at PC.
Identifiants
pubmed: 30919360
doi: 10.1007/978-1-4939-9158-7_17
doi:
Substances chimiques
beta-Arrestin 2
0
Green Fluorescent Proteins
147336-22-9
Somatostatin
51110-01-1
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM