Generation of Nppa-tagBFP reporter knock-in mouse line for studying cardiac chamber specification.
Nppa
atrium
cardiomyocyte
heart
ventricle
Journal
Genesis (New York, N.Y. : 2000)
ISSN: 1526-968X
Titre abrégé: Genesis
Pays: United States
ID NLM: 100931242
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
20
01
2019
revised:
14
03
2019
accepted:
15
03
2019
pubmed:
29
3
2019
medline:
7
1
2020
entrez:
29
3
2019
Statut:
ppublish
Résumé
Nppa is a cardiac hormone which plays critical roles in regulating salt-water balance. Its expression is restricted to the atria of the healthy post-natal heart. During heart development, spatio-temporal expression of Nppa is dynamically changed within the heart and becomes restricted to the atria upon birth. In contrast to its atrial specific expression after birth, Nppa is re-expressed in the adult ventricles in response to cardiac hypertrophy. To study cardiac chamber specification during development and pathological cardiac remodeling during heart disease, we generated a novel Nppa reporter mouse line by knocking-in a tagBFP reporter cassette into 3'-UTR of the Nppa gene without disrupting the endogenous gene. Our results demonstrated dynamic tagBFP expression in the developing heart, recapitulating the spatiotemporal expression pattern of endogenous Nppa. We also found that Nppa-tagBFP is induced in the ventricle during pathological remodeling. Taken together, Nppa-tagBFP reporter knock-in mouse model described in this article will serve as a valuable tool to study cardiac chamber specification during development as well as pathological cardiac remodeling.
Identifiants
pubmed: 30920727
doi: 10.1002/dvg.23294
pmc: PMC6826257
mid: NIHMS1056965
doi:
Substances chimiques
Nppa protein, mouse
0
Atrial Natriuretic Factor
85637-73-6
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e23294Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL119234
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL133290
Pays : United States
Organisme : NHLBI NIH HHS
ID : R03 HL140264
Pays : United States
Organisme : NIH HHS
ID : S10 OD021630
Pays : United States
Informations de copyright
© 2019 Wiley Periodicals, Inc.
Références
Development. 2014 Nov;141(22):4267-78
pubmed: 25344074
Nat Rev Genet. 2005 Nov;6(11):826-35
pubmed: 16304598
Genes Dev. 2002 May 15;16(10):1234-46
pubmed: 12023302
Dev Biol. 2000 Jul 15;223(2):266-78
pubmed: 10882515
FASEB J. 1991 Dec;5(15):3037-46
pubmed: 1835945
Biochim Biophys Acta. 2013 Apr;1833(4):783-94
pubmed: 23063530
J Clin Invest. 1995 Sep;96(3):1311-8
pubmed: 7657806
Circ Res. 2010 Dec 10;107(12):1445-53
pubmed: 20966393
Genesis. 2003 Sep;37(1):1-4
pubmed: 14502570
Science. 2006 Sep 29;313(5795):1922-7
pubmed: 17008524
Cell. 1986 Dec 5;47(5):695-702
pubmed: 2946416
Circ Res. 2010 Dec 10;107(12):1428-44
pubmed: 21148449
Can J Physiol Pharmacol. 1991 Oct;69(10):1486-92
pubmed: 1723347
J Clin Invest. 2010 Jul;120(7):2280-91
pubmed: 20516643
Genesis. 2018 Oct;56(10):e23256
pubmed: 30307112