The meiotic phosphatase GSP-2/PP1 promotes germline immortality and small RNA-mediated genome silencing.
Animals
Caenorhabditis elegans
/ genetics
Caenorhabditis elegans Proteins
/ genetics
Chromosomal Proteins, Non-Histone
/ metabolism
Chromosome Segregation
Genome
Germ Cells
/ metabolism
Meiosis
/ physiology
Meiotic Prophase I
/ physiology
Methylation
Phosphoric Monoester Hydrolases
Protein Phosphatase 1
/ metabolism
RNA Interference
/ physiology
RNA, Small Interfering
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
28
02
2018
accepted:
05
02
2019
revised:
09
04
2019
pubmed:
29
3
2019
medline:
30
4
2019
entrez:
29
3
2019
Statut:
epublish
Résumé
Germ cell immortality, or transgenerational maintenance of the germ line, could be promoted by mechanisms that could occur in either mitotic or meiotic germ cells. Here we report for the first time that the GSP-2 PP1/Glc7 phosphatase promotes germ cell immortality. Small RNA-induced genome silencing is known to promote germ cell immortality, and we identified a separation-of-function allele of C. elegans gsp-2 that is compromised for germ cell immortality and is also defective for small RNA-induced genome silencing and meiotic but not mitotic chromosome segregation. Previous work has shown that GSP-2 is recruited to meiotic chromosomes by LAB-1, which also promoted germ cell immortality. At the generation of sterility, gsp-2 and lab-1 mutant adults displayed germline degeneration, univalents, histone methylation and histone phosphorylation defects in oocytes, phenotypes that mirror those observed in sterile small RNA-mediated genome silencing mutants. Our data suggest that a meiosis-specific function of GSP-2 ties small RNA-mediated silencing of the epigenome to germ cell immortality. We also show that transgenerational epigenomic silencing at hemizygous genetic elements requires the GSP-2 phosphatase, suggesting a functional link to small RNAs. Given that LAB-1 localizes to the interface between homologous chromosomes during pachytene, we hypothesize that small localized discontinuities at this interface could promote genomic silencing in a manner that depends on small RNAs and the GSP-2 phosphatase.
Identifiants
pubmed: 30921322
doi: 10.1371/journal.pgen.1008004
pii: PGENETICS-D-18-00406
pmc: PMC6456222
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Chromosomal Proteins, Non-Histone
0
LAB-1 protein, C elegans
0
RNA, Small Interfering
0
Protein Phosphatase 1
EC 3.1.3.16
Phosphoric Monoester Hydrolases
EC 3.1.3.2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008004Subventions
Organisme : NIGMS NIH HHS
ID : F32 GM120809
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM083048
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007133
Pays : United States
Organisme : Medical Research Council
ID : A652-5PZ80
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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