Quantitative analysis of microcystin variants by capillary electrophoresis mass spectrometry with dynamic pH barrage junction focusing.
CE-MS interface
capillary electrophoresis
dynamic pH junction
mass spectrometry
microcystins
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
17
01
2019
revised:
04
03
2019
accepted:
24
03
2019
pubmed:
30
3
2019
medline:
5
3
2020
entrez:
30
3
2019
Statut:
ppublish
Résumé
Dynamic pH junction is an online focusing method in CE based on the electrophoretic mobility difference of analytes in the sample matrix and the background electrolyte. An advantage of this method over the conventional CE is that the sensitivity can be significantly improved. By injecting a long sample plug in the capillary and focusing the analytes at the pH boundary between the background electrolyte and sample matrix, the LOD can be improved by 10-100 folds. The dynamic pH junction method can be easily coupled with ESI-MS. In this work, we used this method for the analysis of microcystins (MCs). The detection limits and dynamic ranges were studied. The separation was optimized by adjusting the injection time, and concentrations and pH values of the background electrolyte. The optimization of analyte focusing leads to enhanced detection response compared to conventional injections, achieving 200-400 fold higher averaged peak heights for four microcystin (MC) variants. More importantly, this method was successfully used for the quantitative analysis of microcystins (MCs) in crude algae samples from natural water bodies, making it promising for practical applications.
Identifiants
pubmed: 30924152
doi: 10.1002/elps.201900042
doi:
Substances chimiques
Microcystins
0
Methanol
Y4S76JWI15
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2285-2293Subventions
Organisme : National Natural Science Foundation of China
ID : 21475061
Pays : International
Organisme : Nanjing Science and Technology Plan
ID : 201505008
Pays : International
Organisme : Nanjing Qixia Innovation Fund
ID : GC201801
Pays : International
Organisme : Nanjing Qixia Innovation Fund
ID : ZY201813
Pays : International
Informations de copyright
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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