DNA methylation and functional characterization of the XIST gene during
Animals
Biomarkers
/ analysis
Cattle
DNA Methylation
Embryo, Mammalian
/ cytology
Embryonic Development
/ genetics
Female
Gene Expression Regulation, Developmental
Germ Cells
/ cytology
In Vitro Techniques
Oocytes
/ cytology
Oogenesis
/ genetics
Promoter Regions, Genetic
RNA, Long Noncoding
/ genetics
Single-Cell Analysis
None
DNA methylation
X chromosome inactivation
XIST
gene expression
genomic
Journal
Epigenetics
ISSN: 1559-2308
Titre abrégé: Epigenetics
Pays: United States
ID NLM: 101265293
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
pubmed:
31
3
2019
medline:
29
5
2020
entrez:
31
3
2019
Statut:
ppublish
Résumé
XIST, in association with the shorter ncRNA RepA, are essential for the initiation of X chromosome inactivation (XCI) in mice. The molecular mechanisms controlling XIST and RepA expression are well characterized in that specie. However, little is known in livestock. We aimed to characterize the DNA methylation status along the 5' portion of XIST and to characterize its transcriptional profile during early development in cattle. Three genomic regions of XIST named here as promoter, RepA and DMR1 had their DNA methylation status characterized in gametes and embryos. Expression profile of XIST was evaluated, including sense and antisense transcription. Oocytes showed higher levels of methylation than spermatozoa that was demethylated. DMR1 was hypermethylated throughout oogenesis. At the 8-16-cell embryo stage DMR1 was completed demethylated. Interestingly, RepA gain methylation during oocyte maturation and was demethylated at the blastocyst stage, later than DMR1. These results suggest that DMR1 and RepA are transient differentially methylated regions in cattle. XIST RNA was detected in matured oocytes and in single cells from the 2-cell to the morula stage, confirming the presence of maternal and embryonic transcripts. Sense and antisense transcripts were detected along the XIST in blastocyst.
Identifiants
pubmed: 30925851
doi: 10.1080/15592294.2019.1600828
pmc: PMC6557597
doi:
Substances chimiques
Biomarkers
0
RNA, Long Noncoding
0
XIST non-coding RNA
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
568-588Références
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