Nef-mediated inhibition of NFAT following TCR stimulation differs between HIV-1 subtypes.


Journal

Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674

Informations de publication

Date de publication:
05 2019
Historique:
received: 13 12 2018
revised: 13 02 2019
accepted: 16 02 2019
pubmed: 31 3 2019
medline: 23 10 2019
entrez: 31 3 2019
Statut: ppublish

Résumé

Functional characterisation of different HIV-1 subtypes may improve understanding of viral pathogenesis and spread. Here, we evaluated the ability of 345 unique HIV-1 Nef clones representing subtypes A, B, C and D to inhibit NFAT signalling following TCR stimulation. The contribution of this Nef function to disease progression was also assessed in 211 additional Nef clones isolated from unique subtype C infected individuals in early or chronic infection. On average, subtype A and C Nef clones exhibited significantly lower ability to inhibit TCR-mediated NFAT signalling compared to subtype B and D Nef clones. While this observation corroborates accumulating evidence supporting relative attenuation of subtypes A and C that may paradoxically contribute to their increased global prevalence and spread, no significant correlations between Nef-mediated NFAT inhibition activity and clinical markers of HIV-1 infection were observed, indicating that the relationship between Nef function and pathogenesis is complex.

Identifiants

pubmed: 30927712
pii: S0042-6822(19)30057-1
doi: 10.1016/j.virol.2019.02.011
pmc: PMC6526282
mid: NIHMS1525613
pii:
doi:

Substances chimiques

NFATC Transcription Factors 0
Receptors, Antigen, T-Cell 0
nef Gene Products, Human Immunodeficiency Virus 0
nef protein, Human immunodeficiency virus 1 0

Types de publication

Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

192-202

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : R37 AI067073
Pays : United States
Organisme : NIAID NIH HHS
ID : UM1 AI126617
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

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Auteurs

Lisa Naidoo (L)

HIV Pathogenesis Programme, University of KwaZulu-Natal, Durban 4001, South Africa.

Zinhle Mzobe (Z)

HIV Pathogenesis Programme, University of KwaZulu-Natal, Durban 4001, South Africa.

Steven W Jin (SW)

Faculty of Health Sciences, Simon Fraser University, Burnaby, BC, Canada V5A 1S6.

Erasha Rajkoomar (E)

HIV Pathogenesis Programme, University of KwaZulu-Natal, Durban 4001, South Africa.

Tarylee Reddy (T)

Medical Research Council, Biostatistics Unit, Durban 4001, South Africa.

Mark A Brockman (MA)

Faculty of Health Sciences, Simon Fraser University, Burnaby, BC, Canada V5A 1S6; Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada V5A 1S6; British Columbia Centre for Excellence in HIV/AIDS, Vancouver, BC, Canada V6Z 1Y6.

Zabrina L Brumme (ZL)

Faculty of Health Sciences, Simon Fraser University, Burnaby, BC, Canada V5A 1S6; British Columbia Centre for Excellence in HIV/AIDS, Vancouver, BC, Canada V6Z 1Y6.

Thumbi Ndung'u (T)

HIV Pathogenesis Programme, University of KwaZulu-Natal, Durban 4001, South Africa; Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard University, Cambridge, MA, USA; Africa Health Research Institute, Durban 4001, South Africa; Max Planck Institute for Infection Biology, Chariteplatz, D-10117 Berlin, Germany.

Jaclyn K Mann (JK)

HIV Pathogenesis Programme, University of KwaZulu-Natal, Durban 4001, South Africa. Electronic address: mannj@ukzn.ac.za.

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