Hypoxia-induced human deoxyribonuclease I is a cellular restriction factor of hepatitis B virus.
Cell Line
Cobalt
/ pharmacology
DNA, Viral
/ metabolism
Deoxyribonuclease I
/ genetics
Gene Expression
Gene Expression Regulation, Enzymologic
/ drug effects
Hepatitis B
/ enzymology
Hepatitis B Core Antigens
/ metabolism
Hepatitis B virus
/ physiology
Humans
Hypoxia
/ chemically induced
Hypoxia-Inducible Factor 1
/ metabolism
Liver Cirrhosis
/ enzymology
Mutation
Virion
/ metabolism
Virus Replication
/ drug effects
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
20
09
2017
accepted:
11
02
2019
pubmed:
3
4
2019
medline:
14
9
2019
entrez:
3
4
2019
Statut:
ppublish
Résumé
Numerous human APOBEC3 cytidine deaminases have proven to be, inter alia, host cell restriction factors for retroviruses and hepadnaviruses. Although they can bind to genomic RNA and become encapsidated, they are only catalytically active on single-stranded DNA. As there are many cellular deoxyribonucleases (DNases), we hypothesized that a parallel could be struck between APOBEC3 and DNases. For human hepatitis B virus (HBV), we show that DNase I can considerably reduce the virion genome copy number from a variety of transfected or infected cells. DNASE1 is overexpressed and encapsidated in HBV particles in vitro in hypoxic environments and in vivo in cirrhotic patient livers as well as in the serum of infected patients. The use of CoCl
Identifiants
pubmed: 30936483
doi: 10.1038/s41564-019-0405-x
pii: 10.1038/s41564-019-0405-x
doi:
Substances chimiques
DNA, Viral
0
Hepatitis B Core Antigens
0
Hypoxia-Inducible Factor 1
0
Cobalt
3G0H8C9362
DNASE1 protein, human
EC 3.1.21.1
Deoxyribonuclease I
EC 3.1.21.1
cobaltous chloride
EVS87XF13W
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1196-1207Références
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