Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strains.
Aminoglycosides
/ pharmacology
Amoxicillin
/ pharmacology
Anti-Bacterial Agents
/ pharmacology
Ciprofloxacin
/ pharmacology
Drug Resistance, Multiple, Bacterial
/ genetics
Escherichia coli Infections
/ microbiology
Escherichia coli Proteins
/ genetics
Humans
Microbial Sensitivity Tests
Phenotype
Urinary Tract Infections
/ microbiology
Uropathogenic Escherichia coli
/ drug effects
Virulence
Virulence Factors
/ genetics
Uropathogenic Strains (UPEC)
antibiotics
biofilm
virulence factor genes
Journal
Virulence
ISSN: 2150-5608
Titre abrégé: Virulence
Pays: United States
ID NLM: 101531386
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
entrez:
3
4
2019
pubmed:
3
4
2019
medline:
7
3
2020
Statut:
ppublish
Résumé
Antibiotic therapy and its consequences in bacterial and human aspects are widely investigated. Despite this, the emergence of new multidrug resistant bacteria is still a current problem. The scope of our work included the observation of changes among uropathogenic Escherichia coli strains after the treatment with a subinhibitory concentration of different antibiotics. The sensitive strains with or without virulence factors were incubated with amoxicillin, ciprofloxacin, gentamycin, or tobramycin. After each passage, the E. coli derivatives were compared to their wild types based on their susceptibility profiles, virulence genes, biofilm formations and the fingerprint profiles of PCR products amplified with using the (N)(6)(CGG)(4) primer. It turned out that antibiotics caused significant changes in the repertoire of bacterial virulence and biofilm formation, corresponding to acquired cross-resistance. The genomic changes among the studied bacteria were reflected in the changed profiles of the CGG-PCR products. In conclusion, the inappropriate application of antibiotics may cause a rapid rise of Multidrug Resistant (MDR) strains and give bacteria a chance to modulate their own pathogenicity. This phenomenon has been easily observed among uropathogenic E. coli strains and it is one of the main reasons for recurrent infections of the urinary tract.
Identifiants
pubmed: 30938219
doi: 10.1080/21505594.2019.1596507
pmc: PMC6527016
doi:
Substances chimiques
Aminoglycosides
0
Anti-Bacterial Agents
0
Escherichia coli Proteins
0
Virulence Factors
0
Ciprofloxacin
5E8K9I0O4U
Amoxicillin
804826J2HU
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
260-276Références
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