Tailoring a robust and recyclable nanobiocatalyst by immobilization of Pseudomonas fluorescens lipase on carbon nanofiber and its application in synthesis of enantiopure carboetomidate analogue.
Carbon nanofiber (CNF)
Covalent immobilization
Mitsunobu reaction
Nanobiocatalyst
Pseudomonas fluorescens lipase (PFL)
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
15 Jul 2019
15 Jul 2019
Historique:
received:
06
01
2019
revised:
22
03
2019
accepted:
29
03
2019
pubmed:
4
4
2019
medline:
21
11
2019
entrez:
4
4
2019
Statut:
ppublish
Résumé
Pseudomonas fluorescens lipase (PFL) was covalently immobilized on carbon nanofiber (CNF) using 1‑ethyl‑3‑[3‑dimethylaminopropyl] carbodiimide (EDC)/N‑hydroxysuccinimide (NHS). Surface functionalization of carbon nanofiber augments dispersibility as well as efficiency of covalent immobilization. Crucial parameters for immobilization such as pH, enzyme-support ratio, reaction time and mixing rate were optimized using one factor at a time (OFAT) approach. The nanobiocatalyst prepared under optimized conditions demonstrated a ten-fold increase in enzyme activity and the advantage of high thermal stability (up to 85 °C) along with 10 cycles of reusability. Subsequently practical application of the nanobiocatalyst was explored in the kinetic resolution of racemic 1‑phenylethanol into (S)‑1‑phenylethanol [C = 49.1%, ee
Identifiants
pubmed: 30940586
pii: S0141-8130(19)30138-2
doi: 10.1016/j.ijbiomac.2019.03.231
pii:
doi:
Substances chimiques
Enzymes, Immobilized
0
Pyrroles
0
carboetomidate
0
Carbon
7440-44-0
Lipase
EC 3.1.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1299-1310Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.