Real-time functional analysis of Hv1 channel in neutrophils: a new approach from zebrafish model.
Animals
Animals, Genetically Modified
Calcium Signaling
Immunity, Innate
Ion Channel Gating
Ion Channels
/ deficiency
Membrane Potentials
Neutrophils
/ immunology
Phagocytosis
Phagosomes
/ immunology
Reactive Oxygen Species
/ metabolism
Time Factors
Zebrafish
/ embryology
Zebrafish Proteins
/ deficiency
in vivo model
phagocytosis
proton channel
reactive oxygen species
zebrafish
Journal
American journal of physiology. Regulatory, integrative and comparative physiology
ISSN: 1522-1490
Titre abrégé: Am J Physiol Regul Integr Comp Physiol
Pays: United States
ID NLM: 100901230
Informations de publication
Date de publication:
01 06 2019
01 06 2019
Historique:
pubmed:
4
4
2019
medline:
20
2
2020
entrez:
4
4
2019
Statut:
ppublish
Résumé
Voltage-gated proton channel (Hv1) has been studied in various immune cells, including neutrophils. However, most studies have taken an in vitro approach using isolated cells or primary cultured cells of mammals; therefore, limited evidence is available on the function of Hv1 in a physiological context. In this study, we have developed the in vivo system that enables real-time functional analysis of Hv1 using zebrafish embryos (
Identifiants
pubmed: 30943046
doi: 10.1152/ajpregu.00326.2018
doi:
Substances chimiques
Ion Channels
0
Reactive Oxygen Species
0
Zebrafish Proteins
0
hvcn1 protein, zebrafish
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM