Intestinal Response to Acute Intragastric and Intravenous Administration of Phosphate in Rats.
Adaption
Intestine
Phosphate
Transporter
Journal
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
ISSN: 1421-9778
Titre abrégé: Cell Physiol Biochem
Pays: Germany
ID NLM: 9113221
Informations de publication
Date de publication:
2019
2019
Historique:
received:
15
03
2018
accepted:
02
04
2019
entrez:
5
4
2019
pubmed:
5
4
2019
medline:
13
4
2019
Statut:
ppublish
Résumé
Phosphate (Pi) homeostasis is controlled by the intestine and kidneys whose capacities to transport Pi are under endocrine control. Several studies point to intestinal absorption as a therapeutic target to modulate Pi homeostasis. The small intestine is responsible for almost all Pi absorption in the gut, a process involving Na Duodenal and jejunal mucosa was collected 40 minutes and/or 4 hours after administration (i.g and i.v) of either NaCl or Pi to anaesthetized rats. Uptakes of Pi and protein expression of Na Pi-loading did not modify transport of Pi in duodenal and jejunal BBMV 4 hours after treatment. Administration of Pi did not alter either the intestinal expression of NaPi-IIb and Pit-2 mRNAs, whereas Pit-1 mRNA expression was only regulated (diminished) in duodenum collected 4 hours after i.g Pi-loading. NaPi-IIb protein expression was decreased in duodenum 4 hours upon i.v Pi infusion, whereas the duodenal and jejunal abundance of the cotransporter was unaffected by i.g administration of Pi. Together, these data suggest that the intestine responds acutely to Pi-loading, though this response seems slower than the renal adaptation.
Sections du résumé
BACKGROUND/AIMS
OBJECTIVE
Phosphate (Pi) homeostasis is controlled by the intestine and kidneys whose capacities to transport Pi are under endocrine control. Several studies point to intestinal absorption as a therapeutic target to modulate Pi homeostasis. The small intestine is responsible for almost all Pi absorption in the gut, a process involving Na
METHODS
METHODS
Duodenal and jejunal mucosa was collected 40 minutes and/or 4 hours after administration (i.g and i.v) of either NaCl or Pi to anaesthetized rats. Uptakes of Pi and protein expression of Na
RESULTS
RESULTS
Pi-loading did not modify transport of Pi in duodenal and jejunal BBMV 4 hours after treatment. Administration of Pi did not alter either the intestinal expression of NaPi-IIb and Pit-2 mRNAs, whereas Pit-1 mRNA expression was only regulated (diminished) in duodenum collected 4 hours after i.g Pi-loading. NaPi-IIb protein expression was decreased in duodenum 4 hours upon i.v Pi infusion, whereas the duodenal and jejunal abundance of the cotransporter was unaffected by i.g administration of Pi.
CONCLUSION
CONCLUSIONS
Together, these data suggest that the intestine responds acutely to Pi-loading, though this response seems slower than the renal adaptation.
Substances chimiques
Phosphates
0
RNA, Messenger
0
Sodium-Phosphate Cotransporter Proteins, Type IIb
0
Transcription Factor Pit-1
0
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
838-849Subventions
Organisme : National Center for Competence in Research (NCCR) Kidney.CH
Pays : Switzerland
Organisme : Aragon Regional Government
Pays : Spain
Organisme : Programa IBERCAJA-CAI
Pays : Spain
Informations de copyright
© Copyright by the Author(s). Published by Cell Physiol Biochem Press.
Déclaration de conflit d'intérêts
The authors declare that they have no competing financial interests.