Thymic Epithelium Abnormalities in DiGeorge and Down Syndrome Patients Contribute to Dysregulation in T Cell Development.


Journal

Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960

Informations de publication

Date de publication:
2019
Historique:
received: 01 10 2018
accepted: 19 02 2019
entrez: 6 4 2019
pubmed: 6 4 2019
medline: 4 9 2020
Statut: epublish

Résumé

The thymus plays a fundamental role in establishing and maintaining central and peripheral tolerance and defects in thymic architecture or AIRE expression result in the development of autoreactive lymphocytes. Patients with partial DiGeorge Syndrome (pDGS) and Down Syndrome (DS) present alterations in size and architecture of the thymus and higher risk to develop autoimmunity. We sought to evaluate thymic architecture and thymocyte development in DGS and DS patients and to determine the extent to which thymic defects result in immune dysregulation and T cell homeostasis perturbation in these patients. Thymi from pediatric patients and age-matched controls were obtained to evaluate cortex and medullary compartments, AIRE expression and thymocyte development. In the same patients we also characterized immunophenotype of peripheral T cells. Phenotypic and functional characterization of thymic and peripheral regulatory T (Treg) cells was finally assessed. Histologic analysis revealed peculiar alterations in thymic medulla size and maturation in DGS and DS patients. Perturbed distribution of thymocytes and altered thymic output was also observed. DGS patients showed lower mature CD4

Identifiants

pubmed: 30949166
doi: 10.3389/fimmu.2019.00447
pmc: PMC6436073
doi:

Types de publication

Clinical Trial Journal Article Multicenter Study Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

447

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Auteurs

Genni Enza Marcovecchio (GE)

Division of Regenerative Medicine, Stem Cells and Gene Therapy, Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, Milan, Italy.
Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy.

Ileana Bortolomai (I)

Division of Regenerative Medicine, Stem Cells and Gene Therapy, Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, Milan, Italy.
The Milan Unit, Istituto di Ricerca Genetica e Biomedica, Consiglio Nazionale delle Ricerche, Milan, Italy.

Francesca Ferrua (F)

Division of Regenerative Medicine, Stem Cells and Gene Therapy, Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, Milan, Italy.
Vita-Salute San Raffaele University, Milan, Italy.
Pediatric Immunohematology and Bone Marrow Transplantation Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Elena Fontana (E)

The Milan Unit, Istituto di Ricerca Genetica e Biomedica, Consiglio Nazionale delle Ricerche, Milan, Italy.
Humanitas Clinical and Research Center, Rozzano, Milan, Italy.

Luisa Imberti (L)

Laboratorio CREA (Centro di Ricerca Emato-oncologica AIL), ASST Spedali Civili of Brescia, Brescia, Italy.

Erika Conforti (E)

Department of Pediatric Cardiac Surgery, IRCCS San Donato Milanese Hospital, San Donato Milanese, Milan, Italy.

Donato Amodio (D)

Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy.
University Department of Pediatrics, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.

Sonia Bergante (S)

Laboratory of Stem Cells for Tissue Engineering, Istituto di Ricovero e Cura a Carattere Scientifico, Policlinico San Donato, Milan, Italy.

Giulia Macchiarulo (G)

Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy.
University Department of Pediatrics, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.

Veronica D'Oria (V)

Department of Pediatric Cardiac Surgery, IRCCS San Donato Milanese Hospital, San Donato Milanese, Milan, Italy.

Francesca Conti (F)

University Department of Pediatrics, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.

Silvia Di Cesare (S)

Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy.

Georgia Fousteri (G)

Division of Immunology Transplantation and Infectious Diseases, Diabetes Research Institute, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Adriano Carotti (A)

Department of Pediatric Cardiac Surgery, IRCCS Bambino Gesú Children's Hospital, Rome, Italy.

Alessandro Giamberti (A)

Department of Congenital Cardiac Surgery, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy.

Pietro Luigi Poliani (PL)

Pathology Unit, Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.

Luigi D Notarangelo (LD)

Laboratory of Clinical Immunology and Microbiology, IDGS, DIR, NIAID, NIH, Bethesda, MD, United States.

Caterina Cancrini (C)

Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy.
University Department of Pediatrics, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.

Anna Villa (A)

Division of Regenerative Medicine, Stem Cells and Gene Therapy, Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, Milan, Italy.
The Milan Unit, Istituto di Ricerca Genetica e Biomedica, Consiglio Nazionale delle Ricerche, Milan, Italy.

Marita Bosticardo (M)

Division of Regenerative Medicine, Stem Cells and Gene Therapy, Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, Milan, Italy.
Laboratory of Clinical Immunology and Microbiology, IDGS, DIR, NIAID, NIH, Bethesda, MD, United States.

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Classifications MeSH