The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification.
Animals
Apoptosis Regulatory Proteins
Cell Differentiation
/ genetics
Cell Lineage
/ genetics
DNA Transposable Elements
/ genetics
DNA-Binding Proteins
Female
Gene Expression Regulation, Developmental
Gene Products, gag
/ genetics
Genomic Imprinting
/ genetics
Heparin-binding EGF-like Growth Factor
/ genetics
Humans
Mice
Nuclear Proteins
/ genetics
Placenta
/ metabolism
Placentation
/ genetics
Pregnancy
RNA-Binding Proteins
/ genetics
Stem Cells
/ cytology
Transcription Factors
/ genetics
Trophoblasts
/ cytology
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
06
03
2019
accepted:
15
03
2019
entrez:
6
4
2019
pubmed:
6
4
2019
medline:
20
12
2019
Statut:
epublish
Résumé
Peg10 (paternally expressed gene 10) is an imprinted gene that is essential for placental development. It is thought to derive from a Ty3-gyspy LTR (long terminal repeat) retrotransposon and retains Gag and Pol-like domains. Here we show that the Gag domain of PEG10 can promote vesicle budding similar to the HIV p24 Gag protein. Expressed in a subset of mouse endocrine organs in addition to the placenta, PEG10 was identified as a substrate of the deubiquitinating enzyme USP9X. Consistent with PEG10 having a critical role in placental development, PEG10-deficient trophoblast stem cells (TSCs) exhibited impaired differentiation into placental lineages. PEG10 expressed in wild-type, differentiating TSCs was bound to many cellular RNAs including Hbegf (Heparin-binding EGF-like growth factor), which is known to play an important role in placentation. Expression of Hbegf was reduced in PEG10-deficient TSCs suggesting that PEG10 might bind to and stabilize RNAs that are critical for normal placental development.
Identifiants
pubmed: 30951545
doi: 10.1371/journal.pone.0214110
pii: PONE-D-19-06605
pmc: PMC6450627
doi:
Substances chimiques
Apoptosis Regulatory Proteins
0
DNA Transposable Elements
0
DNA-Binding Proteins
0
Gene Products, gag
0
Hbegf protein, mouse
0
Heparin-binding EGF-like Growth Factor
0
Nuclear Proteins
0
Peg10 protein, mouse
0
RNA-Binding Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0214110Déclaration de conflit d'intérêts
Authors MA, EV, HB, PL, DSK, RR, SK, JDW, SG, MR, KRA, RJN, MR-G, ZM, KN, and VMD were employees of Genentech. This does not alter our adherence to PLOS ONE policies on sharing data and materials. All reagents generated at Genentech will be available through the Genentech MTA program. There are no patents, products in development or marketed products to declare.
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