Influence of gender, age, shelf-life, and conservation method on the biomechanical behavior of colon tissue under dynamic solicitation.


Journal

Clinical biomechanics (Bristol, Avon)
ISSN: 1879-1271
Titre abrégé: Clin Biomech (Bristol, Avon)
Pays: England
ID NLM: 8611877

Informations de publication

Date de publication:
05 2019
Historique:
received: 04 11 2018
revised: 19 03 2019
accepted: 27 03 2019
pubmed: 8 4 2019
medline: 7 5 2020
entrez: 8 4 2019
Statut: ppublish

Résumé

Data from biomechanical tissue sample studies of the human digestive tract are highly variable. The aim of this study was to investigate 4 factors which could modify the mechanical response of human colonic specimens placed under dynamic solicitation until tissue rupture: gender, age, shelf-life and conservation method. We performed uniaxial dynamic tests of human colonic specimens. Specimens were taken according to three different protocols: refrigerated cadavers without embalming, embalmed cadavers and fresh colonic tissue. A total of 143 specimens were subjected to tensile tests, at a speed of 1 m s Young's modulus of the different conservation protocols are as follows: embalmed, 3.08 ± 1.99; fresh, 2.97 ± 2.59; and refrigerated 3.17 ± 2.05. The type of conservation does not modify the stiffness of the tissue (p = 0.26) but does modify the stress necessary for rupture (p < 0.001) and the strain required to obtain lesions of the outer layer and the inner layer (p < 0.001 and p < 0.05, respectively). Gender is also a factor responsible for a change in the mechanical response of the colon. The age of the subjects and the shelf-life of the bodies did not represent factors influencing the mechanical behavior of the colon (p > 0.05). The mechanical response of the colon tissue showed a biphasic injury process depending on gender and method of preservation. The age and shelf-life of anatomical subjects do not alter the mechanical response of the colon.

Sections du résumé

BACKGROUND
Data from biomechanical tissue sample studies of the human digestive tract are highly variable. The aim of this study was to investigate 4 factors which could modify the mechanical response of human colonic specimens placed under dynamic solicitation until tissue rupture: gender, age, shelf-life and conservation method.
METHODS
We performed uniaxial dynamic tests of human colonic specimens. Specimens were taken according to three different protocols: refrigerated cadavers without embalming, embalmed cadavers and fresh colonic tissue. A total of 143 specimens were subjected to tensile tests, at a speed of 1 m s
FINDINGS
Young's modulus of the different conservation protocols are as follows: embalmed, 3.08 ± 1.99; fresh, 2.97 ± 2.59; and refrigerated 3.17 ± 2.05. The type of conservation does not modify the stiffness of the tissue (p = 0.26) but does modify the stress necessary for rupture (p < 0.001) and the strain required to obtain lesions of the outer layer and the inner layer (p < 0.001 and p < 0.05, respectively). Gender is also a factor responsible for a change in the mechanical response of the colon. The age of the subjects and the shelf-life of the bodies did not represent factors influencing the mechanical behavior of the colon (p > 0.05).
INTERPRETATION
The mechanical response of the colon tissue showed a biphasic injury process depending on gender and method of preservation. The age and shelf-life of anatomical subjects do not alter the mechanical response of the colon.

Identifiants

pubmed: 30954683
pii: S0268-0033(18)30920-3
doi: 10.1016/j.clinbiomech.2019.03.017
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

34-40

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

D Massalou (D)

Emergency Surgery Unit, University Hospital of Nice, CHU de Nice Hôpital Pasteur 2, Université de Nice Sophia-Antipolis, France; Biomechanical Applied Laboratory, UMRT24, IFSTTAR, Aix-Marseille University, France. Electronic address: damienmassalou@gmail.com.

C Masson (C)

Biomechanical Applied Laboratory, UMRT24, IFSTTAR, Aix-Marseille University, France. Electronic address: Catherine.masson@ifsttar.fr.

S Afquir (S)

Biomechanical Applied Laboratory, UMRT24, IFSTTAR, Aix-Marseille University, France.

P Baqué (P)

Emergency Surgery Unit, University Hospital of Nice, CHU de Nice Hôpital Pasteur 2, Université de Nice Sophia-Antipolis, France. Electronic address: baque.p@chu-nice.fr.

P-J Arnoux (PJ)

Biomechanical Applied Laboratory, UMRT24, IFSTTAR, Aix-Marseille University, France. Electronic address: pierre-jean.arnoux@ifsttar.fr.

T Bège (T)

Department of Visceral Surgery, AP-HM Hôpital Nord, Aix-Marseille University, France; Biomechanical Applied Laboratory, UMRT24, IFSTTAR, Aix-Marseille University, France. Electronic address: thierry.bege@ap-hm.fr.

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