Study of target spacing of thermo-sensitive carrier on the activity recovery of immobilized penicillin G acylase.

Drug Carriers / chemistry Enzymes, Immobilized / metabolism Molecular Weight Penicillin Amidase / metabolism Polymers / chemistry Proton Magnetic Resonance Spectroscopy Regression Analysis Temperature
Activity recovery rate Carrier Penicillin G acylase Reactivity ratio Target spacing

Journal

Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Titre abrégé: Colloids Surf B Biointerfaces
Pays: Netherlands
ID NLM: 9315133

Informations de publication

Date de publication:
01 Jul 2019
Historique:
received: 25 01 2019
revised: 11 03 2019
accepted: 27 03 2019
pubmed: 8 4 2019
medline: 21 12 2019
entrez: 8 4 2019
Statut: ppublish

Résumé

The immobilized penicillin G acylase (PGA) is an important industrial catalyst, the activity recovery rate of it directly affects enterprise efficiency. How to improve the enzyme activity recovery rate has been a research focus in this field. Based on the above problems, this work further improved the activity recovery rate by adjusting the target spacing for the first time. Glycidyl methacrylate (GMA) was used as the immobilized target and methyl methacrylate (MMA) as the copolymer monomer. According to the copolymer composition equation of P(MMA-co-GMA), the thermo-sensitive copolymers, PDEA-b-PHEMA-b-P(MMA-co-GMA) with different target spacings, were synthesized rapidly and efficiently via reversible addition-fragmentation chain transfer (RAFT) polymerization method. The error range between the theoretical and actual values of MMA and GMA in the copolymers carrier was (0-4)%, which demonstrated that the reliability of using composition equation to accurately and quickly synthesize copolymers with specific spacing. Studies on the thermo-sensitive showed that the low critical solution temperature (LCST) of the copolymer carrier decreased with the increase of hydrophobic monomer. Most importantly, the activity recovery rate increased with the increase of target spacing, and when the molar ratio of MMA to GMA in the copolymer was 8.75:1, the recovery of activity of immobilized PGA could be up to 63.50%, which was 21.70% higher than that of pure GMA. This work provided an important idea for improving the activity of immobilized PGA.

Identifiants

DOI: 10.1016/j.colsurfb.2019.03.064 PMID: 30954878
pubmed: 30954878
pii: S0927-7765(19)30213-9
doi: 10.1016/j.colsurfb.2019.03.064
pii:
doi:

Substances chimiques

Drug Carriers 0
Enzymes, Immobilized 0
Polymers 0
Penicillin Amidase EC 3.5.1.11

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

153-160

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Ke Li (K)

State Key Laboratory of Gansu Advanced Non-ferrous Metal Materials, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China; School of Material Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China.

Guolei Shan (G)

State Key Laboratory of Gansu Advanced Non-ferrous Metal Materials, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China; School of Material Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China.

Xiaobing Ma (X)

State Key Laboratory of Gansu Advanced Non-ferrous Metal Materials, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China; School of Material Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China.

Xinyu Zhang (X)

State Key Laboratory of Gansu Advanced Non-ferrous Metal Materials, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China; School of Material Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China.

Zhenbin Chen (Z)

State Key Laboratory of Gansu Advanced Non-ferrous Metal Materials, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China; School of Material Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China. Electronic address: zhenbinchen@163.com.

Zhenghua Tang (Z)

Guangzhou Key Laboratory for Surface Chemistry of Energy Materials, New Energy Research Institute, School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, 5100067, China; Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency Disposal, School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, 510006, China.

Zhen Liu (Z)

Department of Physics and Engineering, Frostburg State University, Maryland, 21532, United States. Electronic address: zhenliu8@gmail.com.

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Classifications MeSH

questionsmedicales.fr › Thérapeutique › Traitement médicamenteux › Systèmes de délivrance de médicaments › Vecteurs de médicaments › Study of target spacing of thermo-sensitive...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines immobilisées › Enzymes immobilisées › Study of target spacing of thermo-sensitive...
questionsmedicales.fr › Phénomènes chimiques › Masse moléculaire › Study of target spacing of thermo-sensitive...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Amidohydrolases › Penicillin amidase › Study of target spacing of thermo-sensitive...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Study of target spacing of thermo-sensitive...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectroscopie par résonance magnétique › Spectroscopie par résonance magnétique du proton › Study of target spacing of thermo-sensitive...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Analyse de régression › Study of target spacing of thermo-sensitive...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Study of target spacing of thermo-sensitive...