HPV73 a nonvaccine type causes cervical cancer.
Adult
Aged
Brazil
Cross-Sectional Studies
DNA, Neoplasm
/ genetics
Female
Humans
Middle Aged
Oncogene Proteins, Viral
/ genetics
Papillomaviridae
/ genetics
Papillomavirus Infections
/ complications
Polymerase Chain Reaction
/ methods
RNA, Viral
/ genetics
Uterine Cervical Neoplasms
/ etiology
Virus Integration
/ genetics
HPV
cervical cancer
integration
oncogenic type
transcriptionally active
Journal
International journal of cancer
ISSN: 1097-0215
Titre abrégé: Int J Cancer
Pays: United States
ID NLM: 0042124
Informations de publication
Date de publication:
01 02 2020
01 02 2020
Historique:
received:
09
01
2019
revised:
14
03
2019
accepted:
25
03
2019
pubmed:
10
4
2019
medline:
7
2
2020
entrez:
10
4
2019
Statut:
ppublish
Résumé
HPV73 is classified as possibly oncogenic. It is neither routinely evaluated in HPV screening, nor covered by any of the prophylactic vaccines. We sought to investigate the carcinogenic characteristics of HPV73. Molecular studies were performed on eight cervix cancer biopsy specimens containing HPV73 from a cross-sectional cancer cohort of 590 women referred to the National Cancer Institute in Rio de Janeiro, Brazil. Transcriptional activity of HPV73 was evaluated by detection of spliced transcripts of E6/E6* and E1^E4 in cDNA created from RNA isolated from fresh tissue. Disruption of viral E1 and E2 genes in the tumor DNA was assessed by overlapping PCR amplification. Evaluation of viral integration was performed using a customized capture panel and next-generation sequencing, and an in-house bioinformatic pipeline. HPV73 E6/E6* transcripts were found in 7/7 specimens with available RNA, and three also had HPV73 E1^E4 transcripts. Disruption of E1 and E2 genes was observed in 4/8 specimens. Integration of HPV73 sequences into the cancer cell genomes was identified in all cervix cancer tissues. These results provide evidence that HPV73 is an oncogenic virus that can cause invasive cervix cancer. With current molecular screening and HPV vaccination, not all cervix cancers will be prevented.
Identifiants
pubmed: 30963559
doi: 10.1002/ijc.32315
pmc: PMC6785350
mid: NIHMS1024274
doi:
Substances chimiques
DNA, Neoplasm
0
Oncogene Proteins, Viral
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
731-738Subventions
Organisme : NIAID NIH HHS
ID : P30 AI124414
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA013330
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA078527
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA078527
Pays : United States
Informations de copyright
© 2019 UICC.
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