Sensitization potential and potency of terpene hydroperoxides in the cocultured activation test method.
COCAT
cocultured activation test
fragrances
human skin sensitization potential
hydroperoxides
in vitro
sensitization potency
Journal
Contact dermatitis
ISSN: 1600-0536
Titre abrégé: Contact Dermatitis
Pays: England
ID NLM: 7604950
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
12
12
2018
revised:
14
03
2019
accepted:
07
04
2019
pubmed:
10
4
2019
medline:
7
1
2020
entrez:
10
4
2019
Statut:
ppublish
Résumé
Positive patch test reactions to mixtures of oxidized terpenes containing allergenic hydroperoxides are frequently reported. However, human sensitization data for these hydroperoxides are not available. To analyse and evaluate the human sensitization potential and potency of hydroperoxides in vitro by using human cells. Limonene-1-hydroperoxide, limonene-2-hydroperoxide, citronellol-7-hydroperoxide, cumene hydroperoxide, 1-(1-hydroperoxy-1-methylethyl)cyclohexene and mixtures of citronellol hydroperoxides (isomers at positions 6 and 7) and linalool hydroperoxides (isomers at positions 6 and 7) were studied. All compounds were synthesized except for cumene hydroperoxide, which was commercially available. Their potential and potency to activate dendritic cells (DCs) was evaluated by measuring the upregulation of CD86 and CD54 on THP-1 cells upon exposure in the cocultured activation test (COCAT) consisting of HaCaT cells (human keratinocyte cell line) and THP-1 monocytes (as a surrogate for DCs). Hydroperoxides upregulated CD86 and/or CD54 on cocultured THP-1 cells in a concentration-dependent manner. The results are comparable with their sensitization potency ranking in predictive animal models. For the first time, the human sensitization potential and potency of several hydroperoxides were determined by the use of human cells and the COCAT method.
Sections du résumé
BACKGROUND
BACKGROUND
Positive patch test reactions to mixtures of oxidized terpenes containing allergenic hydroperoxides are frequently reported. However, human sensitization data for these hydroperoxides are not available.
OBJECTIVES
OBJECTIVE
To analyse and evaluate the human sensitization potential and potency of hydroperoxides in vitro by using human cells.
MATERIALS/METHODS
METHODS
Limonene-1-hydroperoxide, limonene-2-hydroperoxide, citronellol-7-hydroperoxide, cumene hydroperoxide, 1-(1-hydroperoxy-1-methylethyl)cyclohexene and mixtures of citronellol hydroperoxides (isomers at positions 6 and 7) and linalool hydroperoxides (isomers at positions 6 and 7) were studied. All compounds were synthesized except for cumene hydroperoxide, which was commercially available. Their potential and potency to activate dendritic cells (DCs) was evaluated by measuring the upregulation of CD86 and CD54 on THP-1 cells upon exposure in the cocultured activation test (COCAT) consisting of HaCaT cells (human keratinocyte cell line) and THP-1 monocytes (as a surrogate for DCs).
RESULTS
RESULTS
Hydroperoxides upregulated CD86 and/or CD54 on cocultured THP-1 cells in a concentration-dependent manner. The results are comparable with their sensitization potency ranking in predictive animal models.
CONCLUSIONS
CONCLUSIONS
For the first time, the human sensitization potential and potency of several hydroperoxides were determined by the use of human cells and the COCAT method.
Substances chimiques
Allergens
0
B7-2 Antigen
0
Biomarkers
0
CD86 protein, human
0
Intercellular Adhesion Molecule-1
126547-89-5
Hydrogen Peroxide
BBX060AN9V
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
97-103Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : DFG 284100702
Organisme : Deutsche Forschungsgemeinschaft
ID : BL360-6
Organisme : Agence Nationale de la Recherche
ID : ANR-15-CE15-0023-01
Informations de copyright
© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.