Axial bonds at the T1 Cu site of Thermus thermophilus SG0.5JP17-16 laccase influence enzymatic properties.

Bacterial Proteins / chemistry Catalysis Kinetics Laccase / chemistry Molecular Conformation Mutagenesis, Site-Directed Protein Conformation Thermus thermophilus / enzymology
axial bond copper kinetic analysis laccase redox potential site-directed mutation

Journal

FEBS open bio
ISSN: 2211-5463
Titre abrégé: FEBS Open Bio
Pays: England
ID NLM: 101580716

Informations de publication

Date de publication:
05 2019
Historique:
received: 11 03 2019
revised: 20 03 2019
accepted: 22 03 2019
pubmed: 10 4 2019
medline: 13 11 2019
entrez: 10 4 2019
Statut: ppublish

Résumé

Laccase is a multi-copper oxidase which oxidizes substrate at the type 1 copper site, simultaneously coupling the reduction of dioxygen to water at the trinuclear copper center. In this study, we used site-directed mutagenesis to study the effect of axial bonds between the metal and amino acid residue side chains in lacTT. Our kinetic and spectral data showed that the replacement of the axial residue with non-coordinating residues resulted in higher efficiency (k

Identifiants

DOI: 10.1002/2211-5463.12633 PMID: 30964606 PMC: PMC6487685
pubmed: 30964606
doi: 10.1002/2211-5463.12633
pmc: PMC6487685
doi:

Substances chimiques

Bacterial Proteins 0
Laccase EC 1.10.3.2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

986-995

Informations de copyright

© 2019 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

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Auteurs

Yanyun Zhu (Y)

Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.
Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.

Yi Zhang (Y)

Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.
Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.

Jiangbo Zhan (J)

Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.
Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.

Ying Lin (Y)

Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.
Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.

Xiaorong Yang (X)

Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.
Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Axial bonds at the T1 Cu site of Thermus...
questionsmedicales.fr › Phénomènes chimiques › Catalyse › Axial bonds at the T1 Cu site of Thermus...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Axial bonds at the T1 Cu site of Thermus...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Laccase › Axial bonds at the T1 Cu site of Thermus...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Axial bonds at the T1 Cu site of Thermus...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Ingénierie des protéines › Mutagenèse dirigée › Axial bonds at the T1 Cu site of Thermus...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Axial bonds at the T1 Cu site of Thermus...
questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Bactéries aérobies à Gram négatif › Bâtonnets et coques aérobies à Gram négatif › Thermus › Thermus thermophilus › Axial bonds at the T1 Cu site of Thermus...