Rapid detection of Bacillus ionophore cereulide in food products.
Amino Acid Sequence
Animals
Bacillus cereus
/ metabolism
Bacterial Toxins
/ analysis
Depsipeptides
/ analysis
Fast Foods
/ microbiology
Food Contamination
/ analysis
Food Microbiology
/ methods
Food Safety
/ methods
Limit of Detection
Milk
/ chemistry
Oryza
/ chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/ methods
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
09 04 2019
09 04 2019
Historique:
received:
24
09
2018
accepted:
22
03
2019
entrez:
11
4
2019
pubmed:
11
4
2019
medline:
7
10
2020
Statut:
epublish
Résumé
Cereulide is a toxic cyclic depsipeptide produced by certain strains of Bacillus cereus found in soil and food products. While some harmless strains of Bacillus are used as probiotic, others can cause nausea and vomiting, and represent an important food safety concern. Current detection methods are time consuming and do not necessarily detect toxic cereulide. Here, we developed a rapid protocol using Matrix Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry that detects the toxin originating from a colony smear of B. cereus. The distinct molecular feature of the toxin peak at m/z 1,191 was clearly identified from bacterial extracts with a limit of detection (LOD) of 30 ng/mL. Final optimisation of the sample preparation was based on cereulide chelating cations to produce the alkali adduct [M + K]
Identifiants
pubmed: 30967595
doi: 10.1038/s41598-019-42167-0
pii: 10.1038/s41598-019-42167-0
pmc: PMC6456620
doi:
Substances chimiques
Bacterial Toxins
0
Depsipeptides
0
cereulide
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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