TGF-β activation impairs fibroblast ability to support adult lung epithelial progenitor cell organoid formation.
Adult Stem Cells
/ drug effects
Aged
Animals
Cell Communication
/ drug effects
Cell Differentiation
/ drug effects
Coculture Techniques
Epithelial Cell Adhesion Molecule
/ genetics
Epithelial Cells
/ drug effects
Female
Fibroblast Growth Factor 7
/ pharmacology
Fibroblasts
/ metabolism
Gene Expression Profiling
Gene Expression Regulation
Hepatocyte Growth Factor
/ pharmacology
Humans
Lung
/ metabolism
Male
Mice
Mice, Inbred C57BL
Middle Aged
Myofibroblasts
/ drug effects
Organoids
/ drug effects
Pulmonary Disease, Chronic Obstructive
/ genetics
Pyridines
/ pharmacology
Pyrimidines
/ pharmacology
Transforming Growth Factor beta
/ metabolism
Wnt Proteins
/ genetics
Wnt Signaling Pathway
beta Catenin
/ genetics
TGF-β, Wnt/β-catenin signaling
lung regeneration/repair
lung stem cells
mesenchymal-epithelial signaling
Journal
American journal of physiology. Lung cellular and molecular physiology
ISSN: 1522-1504
Titre abrégé: Am J Physiol Lung Cell Mol Physiol
Pays: United States
ID NLM: 100901229
Informations de publication
Date de publication:
01 07 2019
01 07 2019
Historique:
pubmed:
11
4
2019
medline:
25
3
2020
entrez:
11
4
2019
Statut:
ppublish
Résumé
Transforming growth factor-β (TGF-β)-induced fibroblast-to-myofibroblast differentiation contributes to remodeling in chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis, but whether this impacts the ability of fibroblasts to support lung epithelial repair remains little explored. We pretreated human lung fibroblasts [primary (phFB) or MRC5 cells] with recombinant human TGF-β to induce myofibroblast differentiation, then cocultured them with adult mouse lung epithelial cell adhesion molecule-positive cells (EpCAM
Identifiants
pubmed: 30969812
doi: 10.1152/ajplung.00400.2018
doi:
Substances chimiques
CTNNB1 protein, human
0
Chir 99021
0
Epithelial Cell Adhesion Molecule
0
FGF7 protein, human
0
HGF protein, human
0
Pyridines
0
Pyrimidines
0
Transforming Growth Factor beta
0
Wnt Proteins
0
beta Catenin
0
Fibroblast Growth Factor 7
126469-10-1
Hepatocyte Growth Factor
67256-21-7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM