Spinal Angulation: A Limitation of the Fetal Lamb Model of Myelomeningocele.
Animals
Disease Models, Animal
Female
Gestational Age
Hindlimb
/ innervation
Humans
Locomotion
Lumbar Vertebrae
/ diagnostic imaging
Magnetic Resonance Imaging
Meningomyelocele
/ diagnostic imaging
Mesenchymal Stem Cell Transplantation
/ adverse effects
Placenta
/ cytology
Predictive Value of Tests
Pregnancy
Prenatal Diagnosis
Sheep, Domestic
Spina bifida
Animal model
Fetal surgery
Magnetic resonance imaging
Myelomeningocele
Placenta
Sheep
Spine curvatures
Stem cells
Journal
Fetal diagnosis and therapy
ISSN: 1421-9964
Titre abrégé: Fetal Diagn Ther
Pays: Switzerland
ID NLM: 9107463
Informations de publication
Date de publication:
2019
2019
Historique:
received:
27
06
2018
accepted:
13
12
2018
pubmed:
11
4
2019
medline:
28
4
2020
entrez:
11
4
2019
Statut:
ppublish
Résumé
The surgically induced fetal lamb model is the most commonly used large animal model of myelomeningocele (MMC) but is subject to variation due to surgical technique during defect creation. Thirty-one fetal lambs underwent creation of the MMC defect, followed by defect repair with either an extracellular matrix (ECM) patch (n = 10) or ECM seeded with placental mesenchymal stromal cells (n = 21). Postnatal hindlimb function was assessed using the Sheep Locomotor Rating (SLR) scale. Postmortem magnetic resonance imaging of the lumbar spine was used to measure the level and degree of spinal angulation, as well as cross-sectional area of remaining vertebral bone. Median level of angulation was between the 2nd and 3rd lumbar vertebrae, with a median angle of 24.3 degrees (interquartile range 16.2-35.3). There was a negative correlation between angulation degree and SLR (r = -0.44, p = 0.013). Degree of angulation also negatively correlated with the normalized cross-sectional area of remaining vertebral bone (r = -0.75, p < 0.0001). Surgical creation of fetal MMC leads to varying severity of spinal angulation in the ovine model, which affects postnatal functional outcomes. Postnatal assessment of spinal angulation aids in standardization of the surgical model of fetal MMC repair.
Identifiants
pubmed: 30970373
pii: 000496201
doi: 10.1159/000496201
pmc: PMC7353925
mid: NIHMS1578068
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
376-384Subventions
Organisme : NINDS NIH HHS
ID : R01 NS100761
Pays : United States
Organisme : NICHD NIH HHS
ID : R03 HD091601
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001860
Pays : United States
Informations de copyright
© 2019 S. Karger AG, Basel.
Références
World J Stem Cells. 2015 Jan 26;7(1):195-207
pubmed: 25621120
J Pediatr Surg. 2017 Oct 12;:
pubmed: 29122293
Neurosurgery. 1993 Jun;32(6):1005-10
pubmed: 8327074
AJP Rep. 2016 Jul;6(3):e309-17
pubmed: 27621952
Stem Cells Dev. 2008 Dec;17(6):1095-107
pubmed: 19006451
Pediatr Surg Int. 2003 May;19(3):162-6
pubmed: 12811478
Stem Cells Transl Med. 2015 Jun;4(6):659-69
pubmed: 25911465
Vet J. 2008 Sep;177(3):324-33
pubmed: 17910925
J Neurotrauma. 1995 Feb;12(1):1-21
pubmed: 7783230
J Pediatr Surg. 2003 Mar;38(3):451-8; discussion 451-8
pubmed: 12632366
Cytotherapy. 2017 Jun;19(6):680-688
pubmed: 28438482
Semin Fetal Neonatal Med. 2010 Feb;15(1):9-14
pubmed: 19540177
J Pediatr Surg. 1995 Jul;30(7):1028-32; discussion 1032-3
pubmed: 7472926
Isr Med Assoc J. 2009 Apr;11(4):209-11
pubmed: 19603592
Biomaterials. 2016 Jan;76:133-43
pubmed: 26520044
J Pediatr Surg. 1996 Mar;31(3):397-402
pubmed: 8708911
Neurosurgery. 1990 Jun;26(6):987-92
pubmed: 2362676
Am J Obstet Gynecol. 2005 Oct;193(4):1456-62
pubmed: 16202740
J Pediatr Surg. 2019 Jan;54(1):75-79
pubmed: 30529115
J Pediatr Surg. 2015 Jul;50(7):1134-6
pubmed: 25783311
J Pediatr Surg. 2015 Apr;50(4):617-21
pubmed: 25840074
N Engl J Med. 2011 Mar 17;364(11):993-1004
pubmed: 21306277