Sumoylation of nucleoprotein (NP) mediated by activation of NADPH oxidase complex is a consequence of oxidative cellular stress during infection by Infectious salmon anemia (ISA) virus necessary to viral progeny.
Animals
Fish Diseases
/ genetics
Fish Proteins
/ genetics
Host-Pathogen Interactions
Isavirus
/ genetics
NADPH Oxidases
/ genetics
Nucleoproteins
/ genetics
Orthomyxoviridae Infections
/ genetics
Oxidative Stress
Reactive Oxygen Species
/ metabolism
Respiratory Burst
Salmon
Small Ubiquitin-Related Modifier Proteins
/ genetics
Sumoylation
Viral Proteins
/ genetics
Virion
/ genetics
ISA virus
Maqui
NADPH oxidase complex
NP
ROS
SUMOylation
Journal
Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
29
01
2019
revised:
19
03
2019
accepted:
19
03
2019
pubmed:
12
4
2019
medline:
23
10
2019
entrez:
12
4
2019
Statut:
ppublish
Résumé
The study evaluated the effects of nucleoprotein viral and the infectious virus in SHK-1 cells. The results show a strong respiratory burst activation and the induction of p47phox, SOD, GLURED, and apoptotic genes. Additionally, the cells alter the profile of SUMOylated proteins by the effect of transfection and infection experiments. In silico analyses show a set of structural motifs in NP susceptible of post-translational modification by the SUMO protein. Interestingly, the inhibition of the NADPH oxidase complex blocked the production of reactive oxygen species and the high level of cellular ROS due to the nucleoprotein and the ISAv. At the same time, the blocking of the p38MAPK signaling pathway and the use of Aristotelia chilensis, decreased viral progeny production. These results suggest that the NP triggers a strong production of ROS and modifying the post-translational profile mediated by SUMO-2/3, a phenomenon that favors the production of new virions.
Identifiants
pubmed: 30974383
pii: S0042-6822(19)30083-2
doi: 10.1016/j.virol.2019.03.012
pii:
doi:
Substances chimiques
Fish Proteins
0
Nucleoproteins
0
Reactive Oxygen Species
0
Small Ubiquitin-Related Modifier Proteins
0
Viral Proteins
0
NADPH Oxidases
EC 1.6.3.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
269-279Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.