Structural insight into TRPV5 channel function and modulation.
TRP channel
calcium
calmodulin
cryo-EM
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
30 04 2019
30 04 2019
Historique:
pubmed:
13
4
2019
medline:
26
3
2020
entrez:
13
4
2019
Statut:
ppublish
Résumé
TRPV5 (transient receptor potential vanilloid 5) is a unique calcium-selective TRP channel essential for calcium homeostasis. Unlike other TRPV channels, TRPV5 and its close homolog, TRPV6, do not exhibit thermosensitivity or ligand-dependent activation but are constitutively open at physiological membrane potentials and modulated by calmodulin (CaM) in a calcium-dependent manner. Here we report high-resolution electron cryomicroscopy structures of truncated and full-length TRPV5 in lipid nanodiscs, as well as of a TRPV5 W583A mutant and TRPV5 in complex with CaM. These structures highlight the mechanism of calcium regulation and reveal a flexible stoichiometry of CaM binding to TRPV5.
Identifiants
pubmed: 30975749
pii: 1820323116
doi: 10.1073/pnas.1820323116
pmc: PMC6500171
doi:
Substances chimiques
Calcium Radioisotopes
0
TRPV Cation Channels
0
Calcium
SY7Q814VUP
Banques de données
PDB
['6O1N', '6O1P', '6O1U', '6O20']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
8869-8878Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM098672
Pays : United States
Organisme : NINDS NIH HHS
ID : R35 NS105038
Pays : United States
Organisme : NIH HHS
ID : S10 OD020054
Pays : United States
Organisme : NIH HHS
ID : S10 OD021741
Pays : United States
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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