Bovine endometrium responds differentially to age-matched short and long conceptuses†.
Animals
Blastocyst
/ cytology
Cattle
Cells, Cultured
Embryo Culture Techniques
/ veterinary
Embryo Implantation
/ physiology
Embryo Transfer
/ methods
Embryo, Mammalian
Endometrium
/ physiology
Female
Fertilization in Vitro
/ veterinary
Gestational Age
Male
Matched-Pair Analysis
Pregnancy
Pregnancy, Animal
cattle
conceptus
endometrium explant
transcriptome profiling
Journal
Biology of reproduction
ISSN: 1529-7268
Titre abrégé: Biol Reprod
Pays: United States
ID NLM: 0207224
Informations de publication
Date de publication:
01 07 2019
01 07 2019
Historique:
received:
28
11
2018
revised:
01
02
2019
accepted:
09
04
2019
pubmed:
13
4
2019
medline:
8
9
2020
entrez:
13
4
2019
Statut:
ppublish
Résumé
This study combined in vitro production of bovine blastocysts, multiple embryo transfer techniques, and a conceptus-endometrial explant co-culture system to test the hypothesis that bovine endometrium exposed to long vs. short day 15 conceptuses would exhibit a different transcriptome profile reflective of potential for successful pregnancy establishment. Bovine endometrial explants collected at the late luteal stage of the estrous cycle were cultured in RPMI medium for 6 h with nothing (control), 100 ng/mL recombinant ovine interferon tau (IFNT), a long day 15 conceptus, or a short day 15 conceptus. Transcriptional profiling of the endometrial explants found that exposure of endometrium to IFNT, long conceptuses, or short conceptuses altered (P < 0.05) expression of 491, 498, and 230 transcripts, respectively, compared to the control. Further analysis revealed three categories of differentially expressed genes (DEG): (i) commonly responsive to exposure to IFNT and conceptuses, irrespective of size (n = 223); (ii) commonly responsive to IFNT and long conceptuses only (n = 168); and genes induced by the presence of a conceptus but independent of IFNT (n = 108). Of those 108 genes, 101 were exclusively induced by long conceptuses and functional analysis revealed that regulation of molecular function, magnesium-ion transmembrane transport, and clathrin coat assembly were the principal gene ontologies associated with these DEG. In conclusion, bovine endometrium responds differently to age-matched conceptuses of varying size in both an IFNT-dependent and -independent manner, which may be reflective of the likelihood of successful pregnancy establishment.
Identifiants
pubmed: 30977805
pii: 5449004
doi: 10.1093/biolre/ioz060
pmc: PMC6614577
doi:
Types de publication
Clinical Trial, Veterinary
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
26-39Subventions
Organisme : NICHD NIH HHS
ID : R01 HD072898
Pays : United States
Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of Society for the Study of Reproduction.
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