Effect of somatostatin on human retinal pigment epithelial cells permeability.
Blood-Retinal Barrier
/ drug effects
Cell Membrane Permeability
Cells, Cultured
Electric Impedance
Fluorescein
/ metabolism
Hormones
/ pharmacology
Humans
Hypoxia
/ metabolism
Immunochemistry
Occludin
/ metabolism
Retinal Pigment Epithelium
/ drug effects
Somatostatin
/ pharmacology
Vascular Endothelial Growth Factor A
/ pharmacology
Zonula Occludens-1 Protein
/ metabolism
Hypoxia
Retinal pigment epithelium
SST
TER
VEGF
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
01
10
2018
revised:
05
03
2019
accepted:
05
04
2019
pubmed:
13
4
2019
medline:
15
2
2020
entrez:
13
4
2019
Statut:
ppublish
Résumé
To assess the effect of somatostatin (SST) on the permeability of human retinal pigment epithelial cells. We conducted two experiments, exposing cells from human-fetal retinal pigment epithelium (hfRPE) cultures to vascular endothelial growth factor (VEGF), with or without SST pretreatment, in one, and to hypoxic conditions, again with or without SST pretreatment, in the other. The paracellular permeability of hfRPE was assessed by measuring transepithelial electrical resistance (TER) and fluorescein isothiocyanate-sodium (FITC-sodium) flux. Immunochemistry analysis was used to assess the expression of occludin and Zonula occludens-1(ZO-1). Both VEGF and hypoxia increased permeability of the hfRPE, as measured by TER and tracer flux, and decreased occludin and ZO-1staining, as measured by immunochemistry. Pretreatment of cultures with SST partially counteracted these effects. Somatostatin may play a role in the regulation of permeability across retinal pigment epithelium. It may act as an anti-permeability factor in the retina through the enhancement of tight junction function.
Identifiants
pubmed: 30978347
pii: S0014-4835(18)30719-X
doi: 10.1016/j.exer.2019.04.007
pii:
doi:
Substances chimiques
Hormones
0
OCLN protein, human
0
Occludin
0
TJP1 protein, human
0
Vascular Endothelial Growth Factor A
0
Zonula Occludens-1 Protein
0
Somatostatin
51110-01-1
Fluorescein
TPY09G7XIR
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15-23Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.