Diversity of dermal fibroblasts as major determinant of variability in cell reprogramming.
Abdomen
/ growth & development
Adult
Apoptosis
Breast
/ cytology
Cell Differentiation
Cell Proliferation
Cells, Cultured
Cellular Reprogramming
Female
Fibroblasts
/ classification
Humans
Induced Pluripotent Stem Cells
/ cytology
Mesenchymal Stem Cells
/ cytology
Neck
/ growth & development
Skin
/ cytology
Thigh
/ growth & development
Transcriptome
cell reprogramming
fibroblast
induced pluripotent stem cells (iPSCs)
mesenchymal stem cells (MSCs)
Journal
Journal of cellular and molecular medicine
ISSN: 1582-4934
Titre abrégé: J Cell Mol Med
Pays: England
ID NLM: 101083777
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
21
06
2018
revised:
22
02
2019
accepted:
24
03
2019
pubmed:
14
4
2019
medline:
30
7
2020
entrez:
14
4
2019
Statut:
ppublish
Résumé
Induced pluripotent stem cells (iPSCs) are adult somatic cells genetically reprogrammed to an embryonic stem cell-like state. Notwithstanding their autologous origin and their potential to differentiate towards cells of all three germ layers, iPSC reprogramming is still affected by low efficiency. As dermal fibroblast is the most used human cell for reprogramming, we hypothesize that the variability in reprogramming is, at least partially, because of the skin fibroblasts used. Human dermal fibroblasts harvested from five different anatomical sites (neck, breast, arm, abdomen and thigh) were cultured and their morphology, proliferation, apoptotic rate, ability to migrate, expression of mesenchymal or epithelial markers, differentiation potential and production of growth factors were evaluated in vitro. Additionally, gene expression analysis was performed by real-time PCR including genes typically expressed by mesenchymal cells. Finally, fibroblasts isolated from different anatomic sites were reprogrammed to iPSCs by integration-free method. Intriguingly, while the morphology of fibroblasts derived from different anatomic sites differed only slightly, other features, known to affect cell reprogramming, varied greatly and in accordance with anatomic site of origin. Accordingly, difference also emerged in fibroblasts readiness to respond to reprogramming and ability to form colonies. Therefore, as fibroblasts derived from different anatomic sites preserve positional memory, it is of great importance to accurately evaluate and select dermal fibroblast population prior to induce reprogramming.
Identifiants
pubmed: 30980516
doi: 10.1111/jcmm.14316
pmc: PMC6533477
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4256-4268Informations de copyright
© 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
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