Human Multilineage 3D Spheroids as a Model of Liver Steatosis and Fibrosis.
NAFLD
NASH
PNPLA3
elafibranor
fatty acids
fibrosis
liraglutide
obeticholic acid
organoids
vitamin E
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
02 Apr 2019
02 Apr 2019
Historique:
received:
25
02
2019
revised:
29
03
2019
accepted:
30
03
2019
entrez:
17
4
2019
pubmed:
17
4
2019
medline:
31
7
2019
Statut:
epublish
Résumé
Non-alcoholic fatty liver disease (NAFLD) is the most common liver disorder in western countries. Despite the high prevalence of NAFLD, the underlying biology of the disease progression is not clear, and there are no approved drugs to treat non-alcoholic steatohepatitis (NASH), the most advanced form of the disease. Thus, there is an urgent need for developing advanced in vitro human cellular systems to study disease mechanisms and drug responses. We attempted to create an organoid system genetically predisposed to NAFLD and to induce steatosis and fibrosis in it by adding free fatty acids. We used multilineage 3D spheroids composed by hepatocytes (HepG2) and hepatic stellate cells (LX-2) with a physiological ratio (24:1). HepG2 and LX-2 cells are homozygotes for the
Identifiants
pubmed: 30986904
pii: ijms20071629
doi: 10.3390/ijms20071629
pmc: PMC6480107
pii:
doi:
Substances chimiques
2-(2,6-dimethyl-4-(3-(4-(methylthio)phenyl)-3-oxo-1-propenyl)phenoxyl)-2-methylpropanoic acid
0
Apolipoproteins B
0
Chalcones
0
Collagen Type I
0
Fatty Acids
0
Propionates
0
Liraglutide
839I73S42A
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Vetenskapsrådet
ID : 2016-01527
Organisme : Hjärt-Lungfonden
ID : 20120533
Organisme : the Swedish state under the agreement between the Swedish government and the county councils, the ALF-agreement
ID : SU 2018-04276
Organisme : Novonordisk Foundation Grant for Excellence in Endocrinology
ID : Excellence Project, 9321-430
Organisme : Swedish Diabetes Foundation
ID : DIA 2017-205
Organisme : Research grant from AstraZeneca
ID : Echo Project, 10033852
Organisme : Wallenberg Academy Fellows from the Knut and Alice Wallenberg Foundation
ID : KAW 2017.0203
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