Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase.
Acetyl Coenzyme A
/ metabolism
Adenosine Triphosphate
/ metabolism
Bacterial Proteins
/ metabolism
Calcium Chloride
/ metabolism
Carbon Dioxide
/ metabolism
Citrate (si)-Synthase
/ metabolism
Citric Acid
/ metabolism
Citric Acid Cycle
Enzyme Activation
Magnesium Chloride
/ metabolism
Synechocystis
/ enzymology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 04 2019
15 04 2019
Historique:
received:
04
01
2019
accepted:
04
04
2019
entrez:
17
4
2019
pubmed:
17
4
2019
medline:
8
10
2020
Statut:
epublish
Résumé
Citrate synthase (CS, EC 2.3.3.1) catalyses the initial reaction of the tricarboxylic acid (TCA) cycle. Although CSs from heterotrophic bacteria have been extensively studied, cyanobacterial CSs are not well-understood. Cyanobacteria can produce various metabolites from carbon dioxide. Synechocystis sp. PCC 6803 (Synechocystis 6803) is a cyanobacterium used to synthesize metabolites through metabolic engineering techniques. The production of acetyl-CoA-derived metabolites in Synechocystis 6803 has been widely examined. However, the biochemical mechanisms of reactions involving acetyl-CoA in Synechocystis 6803 are poorly understood. We characterised the CS from Synechocystis 6803 (SyCS) and compared its characteristics with other bacterial CSs. SyCS catalysed only the generation of citrate, and did not catalyse the cleavage of citrate. It is suggested that SyCS is not related to the reductive TCA cycle. The substrate affinity and turnover number of SyCS were lower than those of CSs from heterotrophic bacteria. SyCS was activated by MgCl
Identifiants
pubmed: 30988396
doi: 10.1038/s41598-019-42659-z
pii: 10.1038/s41598-019-42659-z
pmc: PMC6465352
doi:
Substances chimiques
Bacterial Proteins
0
Magnesium Chloride
02F3473H9O
Carbon Dioxide
142M471B3J
Citric Acid
2968PHW8QP
Acetyl Coenzyme A
72-89-9
Adenosine Triphosphate
8L70Q75FXE
Citrate (si)-Synthase
EC 2.3.3.1
Calcium Chloride
M4I0D6VV5M
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6038Références
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