Two amino acids missing of MtrA resulted in increased erythromycin level and altered phenotypes in Saccharopolyspora erythraea.
ATP-Binding Cassette Transporters
/ genetics
Anti-Bacterial Agents
/ biosynthesis
Bacterial Proteins
/ genetics
Biological Transport
Drug Resistance, Bacterial
Erythromycin
/ biosynthesis
Gene Expression Regulation, Bacterial
Genetic Complementation Test
Mutant Proteins
/ genetics
Phenotype
Saccharopolyspora
/ enzymology
Sequence Deletion
Antibiotic resistance
Erythromycin biosynthesis
MtrA-MtrB
Two-component regulatory system
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
22
01
2019
accepted:
01
04
2019
revised:
31
03
2019
pubmed:
19
4
2019
medline:
15
8
2019
entrez:
19
4
2019
Statut:
ppublish
Résumé
The MtrA-MtrB two-component regulatory system is highly conserved in Actinobacteria and plays crucial roles in cell cycle progression, cell morphology, antibiotic resistance, and osmoprotection. Previously, we revealed that the MtrA protein of Saccharopolyspora erythraea E3 strain (a high erythromycin-producing strain) had a two amino acid (H197 and V198) deletion in the DNA recognition helices of the C-terminal domain compared to the wild type S. erythraea strain NRRL2338. Here, we identified mepA (encoding a membrane protein related to metalloendopeptidases) as an MtrA target gene, and found that deleting the two amino acids in MtrA (MtrA
Identifiants
pubmed: 30997553
doi: 10.1007/s00253-019-09825-9
pii: 10.1007/s00253-019-09825-9
doi:
Substances chimiques
ATP-Binding Cassette Transporters
0
Anti-Bacterial Agents
0
Bacterial Proteins
0
MtrA protein, Bacteria
0
Mutant Proteins
0
Erythromycin
63937KV33D
Types de publication
Journal Article
Langues
eng
Pagination
4539-4548Subventions
Organisme : National Natural Science Foundation of China
ID : 31730004
Organisme : National Natural Science Foundation of China
ID : 21575089