Generation of induced pluripotent stem cells (iPSCs) IRMBi002-A from an Alzheimer's disease patient carrying a D694N mutation in the APP gene.
Journal
Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
24
10
2018
revised:
05
04
2019
accepted:
12
04
2019
pubmed:
21
4
2019
medline:
12
3
2020
entrez:
21
4
2019
Statut:
ppublish
Résumé
Induced pluripotent stem cells (iPSC) were generated from skin fibroblasts obtained from a 58 year-old woman suffering from Alzheimer's disease and carrying a D694N mutation on Amyloid precursor protein (APP). Fibroblasts were reprogrammed into iPSC using the integration-free Sendai Virus which allows the expression of the Yamanaka factors. Verification of their pluripotency was achieved by demonstrating the expression of pluripotency markers and their differentiation potential into the three primary germ layers. The cells have the corresponding mutation and present a normal karyotype. The reported APP-D694N iPSC line may be used to model and study human AD pathology in vitro.
Identifiants
pubmed: 31004935
pii: S1873-5061(19)30068-6
doi: 10.1016/j.scr.2019.101438
pii:
doi:
Substances chimiques
APP protein, human
0
Amyloid beta-Protein Precursor
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
101438Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.