Localization Analysis of Heterophilic Antigen Epitopes of H1N1 Influenza Virus Hemagglutinin.
Antibodies, Monoclonal
/ immunology
Antibodies, Viral
/ immunology
Antigens, Heterophile
/ immunology
Antigens, Viral
/ immunology
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Epitope Mapping
Epitopes
/ immunology
Hemagglutination Inhibition Tests
Hemagglutinin Glycoproteins, Influenza Virus
/ immunology
Humans
Influenza A Virus, H1N1 Subtype
/ immunology
Peptides
/ immunology
Software
H1N1 influenza virus
HA antigen
Heterophilic epitope
Localization
Monoclonal antibody
Journal
Virologica Sinica
ISSN: 1995-820X
Titre abrégé: Virol Sin
Pays: Netherlands
ID NLM: 101514185
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
27
08
2018
accepted:
23
01
2019
pubmed:
26
4
2019
medline:
14
8
2019
entrez:
26
4
2019
Statut:
ppublish
Résumé
Previous studies have indicated that two monoclonal antibodies (mAbs; A1-10 and H1-84) of the hemagglutinin (HA) antigen on the H1N1 influenza virus cross-react with human brain tissue. It has been proposed that there are heterophilic epitopes between the HA protein and human brain tissue (Guo et al. in Immunobiology 220:941-946, 2015). However, characterisation of the two mAbs recognising the heterophilic epitope on HA has not yet been performed. In the present study, the common antigens of influenza virus HA were confirmed using indirect enzyme-linked immunosorbent assays and analysed with DNAMAN software. The epitopes were localized to nine peptides in the influenza virus HA sequence and the distribution of the peptides in the three-dimensional structure of HA was determined using PyMOL software. Key amino acids and variable sequences of the antibodies were identified using abYsis software. The results demonstrated that there were a number of common antigens among the five influenza viruses studied that were recognised by the mAbs. One of the peptides, P2 (LVLWGIHHP
Identifiants
pubmed: 31020574
doi: 10.1007/s12250-019-00100-9
pii: 10.1007/s12250-019-00100-9
pmc: PMC6599510
doi:
Substances chimiques
Antibodies, Monoclonal
0
Antibodies, Viral
0
Antigens, Heterophile
0
Antigens, Viral
0
Epitopes
0
H1N1 virus hemagglutinin
0
Hemagglutinin Glycoproteins, Influenza Virus
0
Peptides
0
Types de publication
Journal Article
Langues
eng
Pagination
306-314Références
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