NMR for sample quality assessment in metabolomics.
Plasma
Pre-analytics
Serum
Urine
Journal
New biotechnology
ISSN: 1876-4347
Titre abrégé: N Biotechnol
Pays: Netherlands
ID NLM: 101465345
Informations de publication
Date de publication:
25 Sep 2019
25 Sep 2019
Historique:
received:
10
10
2018
revised:
15
04
2019
accepted:
21
04
2019
pubmed:
26
4
2019
medline:
18
12
2019
entrez:
26
4
2019
Statut:
ppublish
Résumé
The EU Framework 7 project SPIDIA was the occasion for development of NMR approaches to evaluate the impact of different pre-analytical treatments on the quality of biological samples dedicated to metabolomics. Systematic simulation of different pre-analytical procedures was performed on urine and blood serum and plasma. Here we review the key aspects of these studies that have led to the development of CEN technical specifications, to be translated into ISO/IS in the course of the EU Horizon 2020 project SPIDIA4P. Inspired by the SPIDIA results, follow-up research was performed, extending the analysis to different sample types and to the different effects of long-term storage. The latter activity was in conjunction with the local European da Vinci Biobank. These results (which partially contributed to the ANNEX of CEN/TS 16945"MOLECULAR IN VITRO DIAGNOSTIC EXAMINATIONS - SPECIFICATIONS FOR PRE-EXAMINATION PROCESSES FOR METABOLOMICS IN URINE, VENOUS BLOOD SERUM AND PLASMA") are presented in detail.
Identifiants
pubmed: 31022482
pii: S1871-6784(18)31855-7
doi: 10.1016/j.nbt.2019.04.004
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25-34Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.