Developing a single strain for in vitro salvage synthesis of NAD
Artificial operon
Cascade reaction
In vitro metabolic engineering
NAD+
OGAB
Thermophilic enzyme
Journal
Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812
Informations de publication
Date de publication:
25 Apr 2019
25 Apr 2019
Historique:
received:
22
10
2018
accepted:
22
04
2019
entrez:
27
4
2019
pubmed:
27
4
2019
medline:
27
6
2019
Statut:
epublish
Résumé
Thermostable enzymes have several advantages over their mesophilic counterparts for industrial applications. However, trade-offs such as thermal instability of enzyme substrates or co-factors exist. Nicotinamide adenine dinucleotide (NAD An integrated strain, E. coli DH5α (pBR-CI857, pGETS118-NAD The integrated strain allows preparation of an enzyme cocktail that can solve the problem of NAD
Sections du résumé
BACKGROUND
BACKGROUND
Thermostable enzymes have several advantages over their mesophilic counterparts for industrial applications. However, trade-offs such as thermal instability of enzyme substrates or co-factors exist. Nicotinamide adenine dinucleotide (NAD
RESULTS
RESULTS
An integrated strain, E. coli DH5α (pBR-CI857, pGETS118-NAD
CONCLUSIONS
CONCLUSIONS
The integrated strain allows preparation of an enzyme cocktail that can solve the problem of NAD
Identifiants
pubmed: 31023312
doi: 10.1186/s12934-019-1125-x
pii: 10.1186/s12934-019-1125-x
pmc: PMC6482498
doi:
Substances chimiques
NAD
0U46U6E8UK
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
75Subventions
Organisme : Japan Science and Technology Agency
ID : A-STEP (Stage II)
Organisme : Japan Society for the Promotion of Science
ID : 17K07720
Organisme : Japan Society for the Promotion of Science
ID : 18J00236
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