Cell-Free and Yeast-Based Production of the Malarial Lactate Transporter, PfFNT, Delivers Comparable Yield and Protein Quality.
L-lactate
Pichia pastoris
Plasmodium falciparum
biolayer interferometry
cell-free protein production
inhibitor binding
membrane protein
transport
Journal
Frontiers in pharmacology
ISSN: 1663-9812
Titre abrégé: Front Pharmacol
Pays: Switzerland
ID NLM: 101548923
Informations de publication
Date de publication:
2019
2019
Historique:
received:
23
01
2019
accepted:
26
03
2019
entrez:
27
4
2019
pubmed:
27
4
2019
medline:
27
4
2019
Statut:
epublish
Résumé
Cell-free protein production is an attractive alternative to cell-based expression. Rapid results, small-volume reactions, irrelevance of protein toxicity, flexibility, and openness of the system are strong points in favor of the cell-free system. However, the
Identifiants
pubmed: 31024323
doi: 10.3389/fphar.2019.00375
pmc: PMC6467934
doi:
Types de publication
Journal Article
Langues
eng
Pagination
375Références
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