The Influence of an Ultrasonic Cleaning Protocol for CAD/CAM Abutment Surfaces on Cell Viability and Inflammatory Response
CAD/CAM implant abutments
cell viability
inflammatory response
steam cleaning
ultrasonic cleaning
Journal
In vivo (Athens, Greece)
ISSN: 1791-7549
Titre abrégé: In Vivo
Pays: Greece
ID NLM: 8806809
Informations de publication
Date de publication:
Historique:
received:
14
02
2019
revised:
10
03
2019
accepted:
12
03
2019
entrez:
28
4
2019
pubmed:
28
4
2019
medline:
21
8
2019
Statut:
ppublish
Résumé
To evaluate the effect of an ultrasonic cleaning and disinfection method for CAD/CAM abutment surfaces on cell viability and inflammatory response in vitro. Untreated and manually polished surfaces of CAD/CAM generated titanium and zirconia disks were randomly assigned, either to a 3-step ultrasonic cleaning and disinfection process (test: TiUF, TiPF, ZrUF, ZrPF) or to 30 sec steam cleaning (control: TiUS, TiPS, ZrUS, ZrPS). Pre-cleaning surface analyses using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and surface profilometry were performed. Human gingival fibroblasts (HGFs) were cultured on test and control specimens and subsequently examined for cell viability and inflammatory response. Expression of acute inflammatory cytokine interleukin (IL)-6 and vascular endothelial growth factor A (VEGFA) were assessed by means of RT-qPCR. Cells on all specimens exhibited a satisfactory viability, indicating firm attachment. Cells on polished zirconia samples, cleaned by means of sonication (ZrPF), exhibited significantly higher viability than cells on the same material cleaned by steam (ZrPS), p=0.019. For all other three material/ surface treatment combinations (TiU, TiP, ZrU), no such difference was observed between the cleaning methods. The messenger ribonucleic acid (mRNA) levels of IL-6 and VEGFA were between 50 and 105% of that of the control cells on the non-toxic control surface. mRNA levels of IL-6 and VEGFA correlated well with each other. Except for higher viability of cells cultured on polished zirconia specimens, no universally applicable advantage could be found for the ultrasonic cleaning procedure for zirconia and titanium abutment surfaces regarding cell viability, IL-6 expression or VEGFA expression. The cleaning procedures did not have any negative effect either.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
To evaluate the effect of an ultrasonic cleaning and disinfection method for CAD/CAM abutment surfaces on cell viability and inflammatory response in vitro.
MATERIALS AND METHODS
METHODS
Untreated and manually polished surfaces of CAD/CAM generated titanium and zirconia disks were randomly assigned, either to a 3-step ultrasonic cleaning and disinfection process (test: TiUF, TiPF, ZrUF, ZrPF) or to 30 sec steam cleaning (control: TiUS, TiPS, ZrUS, ZrPS). Pre-cleaning surface analyses using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and surface profilometry were performed. Human gingival fibroblasts (HGFs) were cultured on test and control specimens and subsequently examined for cell viability and inflammatory response. Expression of acute inflammatory cytokine interleukin (IL)-6 and vascular endothelial growth factor A (VEGFA) were assessed by means of RT-qPCR.
RESULTS
RESULTS
Cells on all specimens exhibited a satisfactory viability, indicating firm attachment. Cells on polished zirconia samples, cleaned by means of sonication (ZrPF), exhibited significantly higher viability than cells on the same material cleaned by steam (ZrPS), p=0.019. For all other three material/ surface treatment combinations (TiU, TiP, ZrU), no such difference was observed between the cleaning methods. The messenger ribonucleic acid (mRNA) levels of IL-6 and VEGFA were between 50 and 105% of that of the control cells on the non-toxic control surface. mRNA levels of IL-6 and VEGFA correlated well with each other.
CONCLUSION
CONCLUSIONS
Except for higher viability of cells cultured on polished zirconia specimens, no universally applicable advantage could be found for the ultrasonic cleaning procedure for zirconia and titanium abutment surfaces regarding cell viability, IL-6 expression or VEGFA expression. The cleaning procedures did not have any negative effect either.
Identifiants
pubmed: 31028185
pii: 33/3/689
doi: 10.21873/invivo.11527
pmc: PMC6559919
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Langues
eng
Pagination
689-698Informations de copyright
Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
Références
Int J Oral Maxillofac Implants. 2017 Sep/Oct;32(5):976-984
pubmed: 28906501
Eur J Oral Implantol. 2015 Autumn;8(3):211-2
pubmed: 26355165
Clin Oral Investig. 2014 May;18(4):1111-1118
pubmed: 23996379
J Immunol. 2011 Dec 15;187(12):6491-8
pubmed: 22095717
Acta Biomater. 2014 Jul;10(7):2894-906
pubmed: 24590162
Med Oral Patol Oral Cir Bucal. 2014 Mar 01;19(2):e177-83
pubmed: 24121917
Clin Oral Implants Res. 2013 Jun;24(6):606-11
pubmed: 22587335
J Clin Periodontol. 1999 Oct;26(10):658-63
pubmed: 10522777
Dent Mater. 2011 Jan;27(1):53-60
pubmed: 21122902
J Prosthodont Res. 2016 Jan;60(1):3-11
pubmed: 26725967
J Biomed Mater Res B Appl Biomater. 2012 Feb;100(2):447-56
pubmed: 22121144
Dent Mater. 2013 Nov;29(11):e263-70
pubmed: 24007922
Eur J Oral Implantol. 2015 Autumn;8(3):213
pubmed: 26355166
Eur J Oral Sci. 1998 Jun;106(3):721-64
pubmed: 9672097
Dent Mater. 2018 Jan;34(1):40-57
pubmed: 29029850
Eur J Oral Implantol. 2015 Autumn;8(3):219
pubmed: 26355174
Int J Periodontics Restorative Dent. 2018 Apr 20;38(5):e87–e95
pubmed: 29677228
Eur J Oral Implantol. 2013 Autumn;6(3):251-60
pubmed: 24179979
J Prosthodont Res. 2017 Jul;61(3):233-241
pubmed: 28359872
Int J Oral Maxillofac Implants. 2017 Nov/Dec;32(6):1315-1323
pubmed: 29140375
Int J Periodontics Restorative Dent. 2017 Sep/Oct;37(5):683-690
pubmed: 28817132
J Prosthet Dent. 2012 Oct;108(4):223-30
pubmed: 23031728
J Biomed Mater Res B Appl Biomater. 2012 Jul;100(5):1334-43
pubmed: 22566390
Clin Oral Implants Res. 2014 Mar;25(3):328-336
pubmed: 23210704
J Adv Prosthodont. 2015 Apr;7(2):151-9
pubmed: 25932314
J Dent Res. 2010 Jan;89(1):91-5
pubmed: 19966039
Adv Immunol. 1993;54:1-78
pubmed: 8379461
J Oral Sci. 2017;59(4):533-539
pubmed: 29279567