Loss of Msh2 and a single-radiation hit induce common, genome-wide, and persistent epigenetic changes in the intestine.
Aged
Animals
Case-Control Studies
Chromatin Immunoprecipitation Sequencing
Disease Models, Animal
Epigenesis, Genetic
/ radiation effects
Female
Gene Regulatory Networks
/ radiation effects
Histones
/ metabolism
Humans
Intestinal Neoplasms
/ etiology
Intestines
/ chemistry
Male
Mice
MutS Homolog 2 Protein
/ genetics
Whole Genome Sequencing
Histone H3 methylation
Intestine
Mismatch repair deficiency
Msh2
Radiation
Journal
Clinical epigenetics
ISSN: 1868-7083
Titre abrégé: Clin Epigenetics
Pays: Germany
ID NLM: 101516977
Informations de publication
Date de publication:
27 04 2019
27 04 2019
Historique:
received:
21
08
2018
accepted:
25
02
2019
entrez:
29
4
2019
pubmed:
29
4
2019
medline:
19
3
2020
Statut:
epublish
Résumé
Mismatch repair (MMR)-deficiency increases the risk of colorectal tumorigenesis. To determine whether the tumors develop on a normal or disturbed epigenetic background and how radiation affects this, we quantified genome-wide histone H3 methylation profiles in macroscopic normal intestinal tissue of young radiated and untreated MMR-deficient VCMsh2 Histone H3 methylation increases in Msh2 MMR deficiency results in genome-wide changes in histone H3 methylation profiles preceding tumor development. Similar changes constitute a persistent epigenetic signature of radiation-induced DNA damage.
Sections du résumé
BACKGROUND
Mismatch repair (MMR)-deficiency increases the risk of colorectal tumorigenesis. To determine whether the tumors develop on a normal or disturbed epigenetic background and how radiation affects this, we quantified genome-wide histone H3 methylation profiles in macroscopic normal intestinal tissue of young radiated and untreated MMR-deficient VCMsh2
RESULTS
Histone H3 methylation increases in Msh2
CONCLUSIONS
MMR deficiency results in genome-wide changes in histone H3 methylation profiles preceding tumor development. Similar changes constitute a persistent epigenetic signature of radiation-induced DNA damage.
Identifiants
pubmed: 31029155
doi: 10.1186/s13148-019-0639-8
pii: 10.1186/s13148-019-0639-8
pmc: PMC6486978
doi:
Substances chimiques
Histones
0
MSH2 protein, human
EC 3.6.1.3
MutS Homolog 2 Protein
EC 3.6.1.3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
65Références
Bioinformatics. 2015 Oct 1;31(19):3225-7
pubmed: 26063839
Cancer Cell. 2015 Aug 10;28(2):198-209
pubmed: 26190263
Int J Radiat Biol. 2007 Apr;83(4):245-57
pubmed: 17575952
Cell. 2013 Apr 25;153(3):590-600
pubmed: 23622243
Nature. 2014 Nov 20;515(7527):355-64
pubmed: 25409824
Nat Commun. 2014 Jun 09;5:4091
pubmed: 24909977
Nat Genet. 2007 Feb;39(2):232-6
pubmed: 17200670
Radiat Oncol. 2016 Jul 26;11:94
pubmed: 27455841
J Pathol. 2017 Oct;243(2):242-254
pubmed: 28727142
Genome Biol. 2008;9(9):R137
pubmed: 18798982
Front Genet. 2014 Sep 25;5:329
pubmed: 25309581
DNA Repair (Amst). 2015 Nov;35:19-26
pubmed: 26435054
Mol Cell Biol. 2005 Apr;25(8):3305-16
pubmed: 15798214
Cancer Res. 2017 Jul 1;77(13):3467-3478
pubmed: 28522752
J Mol Cell Biol. 2015 Apr;7(2):92-104
pubmed: 25735597
PLoS One. 2015 Mar 27;10(3):e0121980
pubmed: 25816162
Oncogene. 2016 Jan 21;35(3):301-13
pubmed: 25823024
Nucleic Acids Res. 2017 Jan 4;45(D1):D183-D189
pubmed: 27899595
Nat Protoc. 2012 Sep;7(9):1728-40
pubmed: 22936215
Genes (Basel). 2018 Jan 05;9(1):
pubmed: 29303998
J Biol Chem. 2011 Mar 11;286(10):7983-9
pubmed: 21239496
Cell. 2010 Oct 1;143(1):134-44
pubmed: 20887898
Exp Cell Res. 2014 Nov 15;329(1):110-5
pubmed: 25017099
J Radiat Res. 2018 Apr 1;59(suppl_2):ii18-ii22
pubmed: 29281035
Mutat Res. 2007 Apr 1;617(1-2):147-51
pubmed: 17331550
Genes Dev. 2006 May 1;20(9):1123-36
pubmed: 16618801
J Clin Oncol. 2010 Jul 10;28(20):3380-7
pubmed: 20516444
Nature. 2007 Aug 2;448(7153):553-60
pubmed: 17603471
Oncogene. 2017 May 11;36(19):2750-2761
pubmed: 27941880
Proc Natl Acad Sci U S A. 2011 Jan 11;108(2):540-5
pubmed: 21187428
Gastroenterology. 2010 Mar;138(3):993-1002.e1
pubmed: 19931261
Nat Genet. 2000 May;25(1):25-9
pubmed: 10802651
Blood. 2017 Dec 14;130(24):2631-2641
pubmed: 29018079
Elife. 2014 May 06;3:e02482
pubmed: 24843002
Nucleic Acids Res. 2017 Jun 2;45(10):5770-5784
pubmed: 28334816
Cancer Res. 2009 May 1;69(9):4073-80
pubmed: 19383908
Gastroenterology. 2015 Nov;149(6):1553-1563.e10
pubmed: 26170137
Cell Rep. 2014 Jun 26;7(6):2006-18
pubmed: 24931610
EMBO J. 2016 Jan 4;35(1):6-23
pubmed: 26628622
J Radiat Res. 2015 Jul;56(4):615-22
pubmed: 25832104
Nature. 2006 May 18;441(7091):349-53
pubmed: 16625203
Cancer Treat Rev. 2010 Nov;36(7):518-27
pubmed: 20413225
J Hum Genet. 2013 Jul;58(7):439-45
pubmed: 23739122
Cell Stem Cell. 2016 Feb 4;18(2):203-13
pubmed: 26831517
Mol Cancer. 2017 Feb 1;16(1):29
pubmed: 28148257
Acta Biochim Biophys Sin (Shanghai). 2016 Jul;48(7):603-16
pubmed: 27217472
PLoS Comput Biol. 2009 Sep;5(9):e1000502
pubmed: 19750212
EMBO Rep. 2015 Nov;16(11):1467-81
pubmed: 26474904
Histopathology. 2010 Jan;56(2):167-79
pubmed: 20102395
Database (Oxford). 2011 Jul 23;2011:bar030
pubmed: 21785142