Improvement of K562 Cell Line Transduction by FBS Mediated Attachment to the Cell Culture Plate.
Journal
BioMed research international
ISSN: 2314-6141
Titre abrégé: Biomed Res Int
Pays: United States
ID NLM: 101600173
Informations de publication
Date de publication:
2019
2019
Historique:
received:
28
10
2018
revised:
09
01
2019
accepted:
21
02
2019
entrez:
30
4
2019
pubmed:
30
4
2019
medline:
17
8
2019
Statut:
epublish
Résumé
Lentiviral vectors have been used for gene therapy in the clinical phase in recent years. These vectors provide a tool for gene insertion, deletion, or modification in organisms. The K562 human cell line has been used extensively in hematopoietic research. Despite its broad application, it is hard-to-transfection and transduction. So, this study presents a simple method to increase the transduction efficiency of K562 cells with a low multiplicity of infection (MOI) of the virus particle. For this purpose, 24-well plate was coated by 300
Identifiants
pubmed: 31032368
doi: 10.1155/2019/9540702
pmc: PMC6457364
doi:
Substances chimiques
Culture Media
0
Types de publication
Journal Article
Langues
eng
Pagination
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