Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Mouse Heart Tissues Using Oroboros 2k-Oxygraph.
Intermyofibrillar mitochondria (IFM)
Mitochondria extraction
Mitochondrial oxygen consumption
Mouse heart
Oxygraph
Subsarcolemmal mitochondria (SSM)
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
2
5
2019
pubmed:
2
5
2019
medline:
28
8
2019
Statut:
ppublish
Résumé
In this chapter, we describe detailed protocols for measuring high-resolution respirometry on mitochondria extracted from adult whole mouse heart using the Oroboros 2k-Oxygraph system. The method provides detailed procedures for the preparation of mitochondria and measurement of high-resolution respirometry in response to various respiration inhibitions. The method described in this chapter could discern the different respiration rate on mitochondria extracted from two spatially distinct mitochondrial subpopulations, subsarcolemmal mitochondria (SSM) and intermyofibrillar mitochondria (IFM). These approaches can easily be translated to other cells and tissues.
Identifiants
pubmed: 31041752
doi: 10.1007/978-1-4939-9195-2_19
pmc: PMC7388157
mid: NIHMS1062103
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
237-246Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL135336
Pays : United States
Organisme : NIA NIH HHS
ID : R03 AG055899
Pays : United States
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