Rapid evaluation of bioactive Ti-based surfaces using an in vitro titration method.
Animals
Biocompatible Materials
/ chemistry
Bone-Implant Interface
Calcium
/ chemistry
Cells, Cultured
Female
Materials Testing
/ methods
Models, Animal
Osseointegration
Osteoblasts
Prostheses and Implants
Prosthesis Implantation
Sheep
Surface Properties
Time Factors
Titanium
/ chemistry
Titrimetry
/ methods
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
02 05 2019
02 05 2019
Historique:
received:
03
09
2018
accepted:
16
03
2019
entrez:
4
5
2019
pubmed:
3
5
2019
medline:
14
6
2019
Statut:
epublish
Résumé
The prediction of implant behavior in vivo by the use of easy-to-perform in vitro methods is of great interest in biomaterials research. Simulated body fluids (SBFs) have been proposed and widely used to evaluate the bone-bonding ability of implant materials. In view of its limitations, we report here a rapid in vitro method based on calcium titration for the evaluation of in vivo bioactivity. Using four different titanium surfaces, this method identifies that alkaline treatment is the key process to confer bioactivity to titanium whereas no significant effect from heat treatment is observed. The presence of bioactive titanium surfaces in the solution during calcium titration induces an earlier nucleation of crystalline calcium phosphates and changes the crystallization pathway. The conclusions from this method are also supported by the standard SBF test (ISO 23317), in vitro cell culture tests using osteoblasts and in vivo animal experiments employing a pelvic sheep model.
Identifiants
pubmed: 31048680
doi: 10.1038/s41467-019-09673-1
pii: 10.1038/s41467-019-09673-1
pmc: PMC6497645
doi:
Substances chimiques
Biocompatible Materials
0
Titanium
D1JT611TNE
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2062Références
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