A sexually dimorphic distribution of corticotropin-releasing factor receptor 1 in the paraventricular hypothalamus.
Animals
Cyclic AMP Response Element-Binding Protein
/ metabolism
Estrogen Receptor alpha
/ metabolism
Female
Hypothalamo-Hypophyseal System
/ metabolism
Male
Mice
Mice, Transgenic
Paraventricular Hypothalamic Nucleus
/ metabolism
Phosphorylation
Pituitary-Adrenal System
/ metabolism
Receptors, Corticotropin-Releasing Hormone
/ metabolism
Restraint, Physical
Sex Characteristics
Stress, Psychological
/ metabolism
androgen
corticotropin releasing factor
sex difference
stress
Journal
Neuroscience
ISSN: 1873-7544
Titre abrégé: Neuroscience
Pays: United States
ID NLM: 7605074
Informations de publication
Date de publication:
15 06 2019
15 06 2019
Historique:
received:
15
01
2019
revised:
08
04
2019
accepted:
23
04
2019
pubmed:
6
5
2019
medline:
10
1
2020
entrez:
6
5
2019
Statut:
ppublish
Résumé
Sex differences in neural structures are generally believed to underlie sex differences reported in anxiety, depression, and the hypothalamic-pituitary-adrenal axis, although the specific circuitry involved is largely unclear. Using a corticotropin-releasing factor receptor 1 (CRFR1) reporter mouse line, we report a sexually dimorphic distribution of CRFR1 expressing cells within the paraventricular hypothalamus (PVN; males > females). Relative to adult levels, PVN CRFR1-expressing cells are sparse and not sexually dimorphic at postnatal days 0, 4, or 21. This suggests that PVN cells might recruit CRFR1 during puberty or early adulthood in a sex-specific manner. The adult sex difference in PVN CRFR1 persists in old mice (20-24 months). Adult gonadectomy (6 weeks) resulted in a significant decrease in CRFR1-immunoreactive cells in the male but not female PVN. CRFR1 cells show moderate co-expression with estrogen receptor alpha (ERα) and high co-expression with androgen receptor, indicating potential mechanisms through which circulating gonadal hormones might regulate CRFR1 expression and function. Finally, we demonstrate that a psychological stressor, restraint stress, induces a sexually dimorphic pattern of neural activation in PVN CRFR1 cells (males >females) as assessed by co-localization with the transcription/neural activation marker phosphorylated CREB. Given the known role of CRFR1 in regulating stress-associated behaviors and hormonal responses, this CRFR1 PVN sex difference might contribute to sex differences in these functions.
Identifiants
pubmed: 31055007
pii: S0306-4522(19)30301-X
doi: 10.1016/j.neuroscience.2019.04.045
pmc: PMC6897333
mid: NIHMS1059927
pii:
doi:
Substances chimiques
Cyclic AMP Response Element-Binding Protein
0
Estrogen Receptor alpha
0
Receptors, Corticotropin-Releasing Hormone
0
CRF receptor type 1
5CLY6W2H1M
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
195-203Subventions
Organisme : NIMH NIH HHS
ID : R01 MH112768
Pays : United States
Organisme : NIMH NIH HHS
ID : R15 MH118692
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2019 IBRO. Published by Elsevier Ltd. All rights reserved.
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