3D Printed Microfluidic Devices for Microchip Electrophoresis of Preterm Birth Biomarkers.
Journal
Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536
Informations de publication
Date de publication:
04 06 2019
04 06 2019
Historique:
pubmed:
7
5
2019
medline:
10
9
2020
entrez:
7
5
2019
Statut:
ppublish
Résumé
This work demonstrates for the first time the creation of microchip electrophoresis devices with ∼50 μm cross-sectional dimensions by stereolithographic 3D printing and their application in the analysis of medically significant biomarkers related to risk for preterm birth (PTB). We determined that device current was linear with applied potential up to 800 V (620 V/cm). We optimized device and separation conditions using fluorescently labeled amino acids as a model system and compared the performance in our 3D printed microfluidic devices to that in other device materials commonly used for microchip electrophoresis analysis. We demonstrated for the first time microchip electrophoresis in a 3D printed device of three PTB biomarkers, including peptides and a protein, with suitable separation characteristics. Limits of detection for microchip electrophoresis in 3D printed microfluidic devices were also determined for PTB biomarkers to be in the high picomolar to low nanomolar range.
Identifiants
pubmed: 31056901
doi: 10.1021/acs.analchem.9b01395
pmc: PMC6561337
mid: NIHMS1027961
doi:
Substances chimiques
Amino Acids
0
Biomarkers
0
Fluorescent Dyes
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
7418-7425Subventions
Organisme : NIBIB NIH HHS
ID : R01 EB006124
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB027096
Pays : United States
Organisme : NIGMS NIH HHS
ID : R15 GM123405
Pays : United States
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