New approach to investigate Common Variable Immunodeficiency patients using spectrochemical analysis of blood.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
10 05 2019
Historique:
received: 14 01 2019
accepted: 04 04 2019
entrez: 12 5 2019
pubmed: 12 5 2019
medline: 21 10 2020
Statut: epublish

Résumé

Common variable immune deficiency (CVID) is a primary immunodeficiency disease, characterized by hypogammaglobulinemia, recurrent infections and various complications. The clinical heterogeneity of CVID has hindered identification of an underlying immune defect; diagnosis relies on clinical judgement, alongside evidence-based criteria. The lack of pathognomonic clinical or laboratory features leads to average diagnostic delays of 5 years or more from the onset. Vibrational spectroscopic techniques such as Fourier-transform infrared (FTIR) spectroscopy have recently gained increasing clinical importance, being rapid-, non-invasive and inexpensive methods to obtain information on the content of biological samples. This has led us to apply FTIR spectroscopy to the investigation of blood samples from a cohort of CVID patients; revealing spectral features capable of stratifying CVID patients from healthy controls with sensitivities and specificities of 97% and 93%, respectively for serum, and 94% and 95%, respectively for plasma. Furthermore we identified several discriminating spectral biomarkers; wavenumbers in regions indicative of nucleic acids (984 cm

Identifiants

pubmed: 31076587
doi: 10.1038/s41598-019-43196-5
pii: 10.1038/s41598-019-43196-5
pmc: PMC6510896
doi:

Substances chimiques

Biomarkers 0

Banques de données

figshare
['10.6084/m9.figshare.7751309']

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

7239

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Auteurs

Emma L Callery (EL)

Department of Immunology, Lancashire Teaching Hospitals NHS Foundation Trust, Preston, PR2 9HT, UK. emma.callery@lthtr.nhs.uk.

Camilo L M Morais (CLM)

School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, PR1 2HE, UK.

Maria Paraskevaidi (M)

School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, PR1 2HE, UK.
Department of Surgery and Cancer, Imperial College London, London, W12 0HS, UK.

Vladimir Brusic (V)

University of Nottingham Ningbo China, Ningbo, 315100, China.

Pavaladurai Vijayadurai (P)

Department of Immunology, Lancashire Teaching Hospitals NHS Foundation Trust, Preston, PR2 9HT, UK.

Ariharan Anantharachagan (A)

Department of Immunology, Lancashire Teaching Hospitals NHS Foundation Trust, Preston, PR2 9HT, UK.

Francis L Martin (FL)

School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, PR1 2HE, UK.

Anthony W Rowbottom (AW)

Department of Immunology, Lancashire Teaching Hospitals NHS Foundation Trust, Preston, PR2 9HT, UK. anthony.rowbottom@lthtr.nhs.uk.
School of Medicine, University of Central Lancashire, Preston, PR1 2HE, UK. anthony.rowbottom@lthtr.nhs.uk.
NIHR Lancashire Clinical Research Facility, Lancashire Teaching Hospitals NHS Foundation Trust, Preston, PR2 9HT, UK. anthony.rowbottom@lthtr.nhs.uk.

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