Diethylstilbestrol induces morphological changes in the spermatogonia, Sertoli cells and Leydig cells of adult rat.
Adult rat
Diethylstibestrol
Leydig cells
Morphology
Sertoli cells
Spermatogonia
Journal
Research in veterinary science
ISSN: 1532-2661
Titre abrégé: Res Vet Sci
Pays: England
ID NLM: 0401300
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
07
08
2018
revised:
28
03
2019
accepted:
22
04
2019
pubmed:
15
5
2019
medline:
9
8
2019
entrez:
15
5
2019
Statut:
ppublish
Résumé
It is now established that diethylstilbestrol (DES) has damaging effects on the male reproductive system. However, to date there have been no studies morphological analysis of adult rat testes upon treatment with DES. Here, we examined whether DES has any significant morphological effect on steroidogenesis and spermatogenesis. DES was injected subcutaneously at 3 μg/day and 30 μg/day in adult male Sprague-Dawley (SD) rats for two different treatment lengths (1 or 3 weeks), after which rats were necropsied. TUNEL labeling, cell counting, and morphological analysis were used to evaluate the effects of DES. A high dose of DES and longer exposure severely affected the cellular development of the testis. Specifically, DES treatment disrupted both steroidogenesis and spermatogenesis by decreasing the number of spermatogonia, Sertoli cells, and Leydig cells in a dose- and time-dependent manner. Thus, DES may account for decreases in the number of spermatogenic cells, Sertoli cells and Leydig cells, which in turn may lead to reduced fertility in males.
Identifiants
pubmed: 31082573
pii: S0034-5288(18)31424-3
doi: 10.1016/j.rvsc.2019.04.015
pii:
doi:
Substances chimiques
Estrogens, Non-Steroidal
0
Diethylstilbestrol
731DCA35BT
Types de publication
Journal Article
Langues
eng
Pagination
433-438Informations de copyright
Copyright © 2019. Published by Elsevier Ltd.