Quantification of staphylococcal enterotoxin type A in cow milk by using a stable isotope-labelled peptide via liquid chromatography-tandem mass spectrometry.

Animals Cattle Chromatography, Liquid Enterotoxins / analysis Food Contamination / analysis Isotope Labeling Milk / chemistry Peptides / chemistry Tandem Mass Spectrometry
Cow milk liquid chromatography–tandem mass spectrometry method validation stable isotope-labelled peptide staphylococcal enterotoxin type A

Journal

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment
ISSN: 1944-0057
Titre abrégé: Food Addit Contam Part A Chem Anal Control Expo Risk Assess
Pays: England
ID NLM: 101485040

Informations de publication

Date de publication:
Jul 2019
Historique:
pubmed: 17 5 2019
medline: 3 8 2019
entrez: 17 5 2019
Statut: ppublish

Résumé

In this study, the staphylococcal enterotoxin type A (SEA) contaminant was quantified in cow milk by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with the use of a stable isotope-labelled peptide of SEA as an internal standard. SEA was cleaned up in a two-step process that included pH control and trichloroacetic acid (TCA) precipitation. The pH control phase eliminated other proteins. TCA precipitation cleaned up SEA without special equipment. An appropriate enzyme-to-protein ratio maximised tryptic digestion. A desalting process guaranteed the stable retention of SEA-digested peptides. The coverage of amino-acid sequences (>10%) clearly identified the toxin's presence. SEA was accurately quantified using LC-MS/MS based on a multiple-reaction monitoring mode. The developed method was validated based on spiked recovery tests at 50 and 100 µg kg

Identifiants

DOI: 10.1080/19440049.2019.1615641 PMID: 31094669
pubmed: 31094669
doi: 10.1080/19440049.2019.1615641
doi:

Substances chimiques

Enterotoxins 0
Peptides 0
enterotoxin A, Staphylococcal 37337-57-8

Types de publication

Journal Article

Langues

eng

Pagination

1098-1108

Auteurs

Hiroshi Koike (H)

a Department of Food Safety , Tokyo Metropolitan Institute of Public Health , Tokyo , Japan.

Maki Kanda (M)

a Department of Food Safety , Tokyo Metropolitan Institute of Public Health , Tokyo , Japan.

Hiroshi Hayashi (H)

a Department of Food Safety , Tokyo Metropolitan Institute of Public Health , Tokyo , Japan.

Yoko Matsushima (Y)

a Department of Food Safety , Tokyo Metropolitan Institute of Public Health , Tokyo , Japan.

Yumi Ohba (Y)

a Department of Food Safety , Tokyo Metropolitan Institute of Public Health , Tokyo , Japan.

Yukiko Nakagawa (Y)

a Department of Food Safety , Tokyo Metropolitan Institute of Public Health , Tokyo , Japan.

Chieko Nagano (C)

a Department of Food Safety , Tokyo Metropolitan Institute of Public Health , Tokyo , Japan.

Kotaro Sekimura (K)

a Department of Food Safety , Tokyo Metropolitan Institute of Public Health , Tokyo , Japan.

Akihiko Hirai (A)

b Department of Microbiology , Tokyo Metropolitan Institute of Public Health , Tokyo , Japan.

Tetsuya Shindo (T)

a Department of Food Safety , Tokyo Metropolitan Institute of Public Health , Tokyo , Japan.

Kenji Otsuka (K)

a Department of Food Safety , Tokyo Metropolitan Institute of Public Health , Tokyo , Japan.

Junichi Kamiie (J)

c Laboratory of Veterinary Pathology, School of Veterinary Medicine , Azabu University , Sagamihara , Japan.

Takeo Sasamoto (T)

a Department of Food Safety , Tokyo Metropolitan Institute of Public Health , Tokyo , Japan.

Tsuneo Hashimoto (T)

a Department of Food Safety , Tokyo Metropolitan Institute of Public Health , Tokyo , Japan.

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Classifications MeSH

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questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Bovins › Quantification of staphylococcal enterotoxin...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Quantification of staphylococcal enterotoxin...
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