Increase in stable isotope ratios driven by metabolic alterations in amphipods exposed to the beta-blocker propranolol.
Adrenergic beta-Antagonists
/ pharmacology
Amphipoda
/ drug effects
Animals
Biomarkers
Carbon Isotopes
/ metabolism
Isotope Labeling
Lipid Peroxidation
/ drug effects
Nitrogen Isotopes
/ metabolism
Oxidation-Reduction
/ drug effects
Principal Component Analysis
Propranolol
/ pharmacology
Thiobarbituric Acid Reactive Substances
/ metabolism
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
10
01
2019
accepted:
05
05
2019
entrez:
17
5
2019
pubmed:
17
5
2019
medline:
14
1
2020
Statut:
epublish
Résumé
Anthropogenic pressures, such as contaminant exposure, may affect stable isotope ratios in biota. These changes are driven by alterations in the nutrient allocation and metabolic pathways induced by specific stressors. In a controlled microcosm study with the amphipod Gammarus spp., we studied effects of the β-blocker propranolol on stable isotope signatures (δ15N and δ13C), elemental composition (%C and %N), and growth (protein content and body size) as well as biomarkers of oxidative status (antioxidant capacity, ORAC; lipid peroxidation, TBARS) and neurological activity (acetylcholinesterase, AChE). Based on the known effects of propranolol exposure on cellular functions, i.e., its mode of action (MOA), we expected to observe a lower scope for growth, accompanied by a decrease in protein deposition, oxidative processes and AChE inhibition, with a resulting increase in the isotopic signatures. The observed responses in growth, biochemical and elemental variables supported most of these predictions. In particular, an increase in %N was observed in the propranolol exposures, whereas both protein allocation and body size declined. Moreover, both ORAC and TBARS levels decreased with increasing propranolol concentration, with the decrease being more pronounced for TBARS, which indicates the prevalence of the antioxidative processes. These changes resulted in a significant increase of the δ15N and δ13C values in the propranolol-exposed animals compared to the control. These findings suggest that MOA of β-blockers may be used to predict sublethal effects in non-target species, including inhibited AChE activity, improved oxidative balance, and elevated stable isotope ratios. The latter also indicates that metabolism-driven responses to environmental contaminants can alter stable isotope signatures, which should be taken into account when interpreting trophic interactions in the food webs.
Identifiants
pubmed: 31095563
doi: 10.1371/journal.pone.0211304
pii: PONE-D-19-00919
pmc: PMC6522046
doi:
Substances chimiques
Adrenergic beta-Antagonists
0
Biomarkers
0
Carbon Isotopes
0
Nitrogen Isotopes
0
Thiobarbituric Acid Reactive Substances
0
Propranolol
9Y8NXQ24VQ
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0211304Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Arch Environ Contam Toxicol. 2002 Aug;43(2):229-35
pubmed: 12115049
Rejuvenation Res. 2007 Mar;10(1):47-59
pubmed: 17378752
Arch Environ Contam Toxicol. 2006 May;50(4):503-10
pubmed: 16583257
Environ Sci Technol. 2016 Aug 2;50(15):8296-304
pubmed: 27367056
J Agric Food Chem. 2001 Oct;49(10):4619-26
pubmed: 11599998
Can J Physiol Pharmacol. 2012 Sep;90(9):1257-68
pubmed: 22913465
Oecologia. 2005 Aug;144(4):534-40
pubmed: 15800751
Anal Bioanal Chem. 2010 Jan;396(2):649-56
pubmed: 19838684
Aquat Toxicol. 2013 Feb;127:46-53
pubmed: 22348951
Environ Sci Technol. 2005 Jun 1;39(11):4226-33
pubmed: 15984804
EXS. 1993;63:98-145
pubmed: 8422542
J Surg Res. 1990 Jan;48(1):38-41
pubmed: 2104945
Clin Sci (Lond). 1981 Jan;60(1):33-40
pubmed: 7237922
Aquat Toxicol. 2009 Aug 31;94(2):114-22
pubmed: 19608286
Biochim Biophys Acta. 1997 Dec 31;1362(2-3):116-27
pubmed: 9540842
Rapid Commun Mass Spectrom. 2003;17(14):1619-25
pubmed: 12845588
Aquat Toxicol. 2004 Mar 30;67(1):45-56
pubmed: 15019250
Exp Biol Med (Maywood). 2006 Apr;231(4):473-84
pubmed: 16565443
PLoS Comput Biol. 2014 Oct 02;10(10):e1003865
pubmed: 25275306
Mar Pollut Bull. 2001 Aug;42(8):656-66
pubmed: 11525283
J Pharm Pharmacol. 1987 Aug;39(8):664-6
pubmed: 2888864
Free Radic Biol Med. 2011 Jan 1;50(1):130-8
pubmed: 20955788
Planta. 2017 Jun;245(6):1067
pubmed: 28456836
Front Pharmacol. 2015 Sep 22;6:205
pubmed: 26441661
Bioessays. 2016 Nov;38(11):1093-1101
pubmed: 27554342
Environ Sci Technol. 2014 Nov 4;48(21):12886-92
pubmed: 25247638
Comp Biochem Physiol A Mol Integr Physiol. 2009 Apr;152(4):470-7
pubmed: 19135164
Oecologia. 2015 Apr;177(4):1211-20
pubmed: 25698143
PLoS One. 2014 Apr 08;9(4):e93774
pubmed: 24713620
Biochem Pharmacol. 1961 Jul;7:88-95
pubmed: 13726518
Aquat Toxicol. 2011 Jan 25;101(2):299-308
pubmed: 21216339
Comp Biochem Physiol A Mol Integr Physiol. 2001 Apr;128(4):791-804
pubmed: 11282322
J Pharm Pharmacol. 1986 Nov;38(11):848-50
pubmed: 2879019
Metabolism. 1988 Jan;37(1):91-8
pubmed: 3275861
Am J Hum Biol. 2013 Jul-Aug;25(4):445-56
pubmed: 23784719
Environ Pollut. 2003;121(2):199-205
pubmed: 12521108
Anal Biochem. 1985 Oct;150(1):76-85
pubmed: 3843705
Comp Biochem Physiol C. 1992 Nov;103(3):563-8
pubmed: 1363306
Basic Res Cardiol. 1988 Jan-Feb;83(1):43-7
pubmed: 2967691
Comp Biochem Physiol B Biochem Mol Biol. 2005 Jan;140(1):81-90
pubmed: 15621513
J Pharmacol Exp Ther. 1975 Feb;192(2):327-35
pubmed: 1117420
Environ Sci Technol. 2008 Aug 1;42(15):5807-13
pubmed: 18754513
Sci Total Environ. 2016 Aug 15;562:777-788
pubmed: 27110989
Aquat Toxicol. 2010 Aug 15;99(2):223-31
pubmed: 20554059
Mol Aspects Med. 2011 Jun;32(3):159-221
pubmed: 21840335
Ecol Evol. 2013 Nov;3(13):4548-57
pubmed: 24340194
Environ Sci Technol. 2015 Jun 2;49(11):6934-42
pubmed: 25893846
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1998 Jan;119(1):97-105
pubmed: 9568379
R Soc Open Sci. 2018 Feb 21;5(2):171398
pubmed: 29515859
Chemosphere. 2009 Aug;76(6):734-9
pubmed: 19576618
Environ Sci Technol. 2018 Apr 17;52(8):4861-4868
pubmed: 29565572
Biochem Biophys Res Commun. 2004 Mar 5;315(2):502-8
pubmed: 14766237