N,N-dimethyltryptamine Prevents Renal Ischemia-Reperfusion Injury in a Rat Model.

Animals Antioxidants / pharmacology Disease Models, Animal Kidney / drug effects Kidney Transplantation / adverse effects Male N,N-Dimethyltryptamine / pharmacology Rats Receptors, sigma / agonists Reperfusion Injury / prevention & control Sigma-1 Receptor

Journal

Transplantation proceedings
ISSN: 1873-2623
Titre abrégé: Transplant Proc
Pays: United States
ID NLM: 0243532

Informations de publication

Date de publication:
May 2019
Historique:
entrez: 19 5 2019
pubmed: 19 5 2019
medline: 27 6 2019
Statut: ppublish

Résumé

Ischemia reperfusion (I/R) injury remains one of the most challenging fields of organ transplantation. It is highly associated with the use of expanded criteria donors that might conclude to delayed graft function or early or late graft failure. To investigate the metabolic, microcirculatory parameters, and histologic changes under the effect of N,N-dimethyltryptamine (DMT) in a renal I/R model in rats. In 26 anesthetized rats both kidneys were exposed. In the control group (n = 6) no other intervention happened. In 20 other animals, the right renal vessels were ligated, and after 60 minutes the right kidney was removed. The left renal vessels were clamped for 60 minutes then released, followed by 120 minutes of reperfusion. In the I/R group (n = 10), there was no additive treatment, while in I/R + DMT group (n = 10) DMT was administered 15 minutes before ischemia. Blood samples were taken, laser Doppler measurement was performed, and both kidneys were evaluated histologically. Microcirculation (blood flux units [BFU]) diminished in all groups, but remarkably so in the I/R + DMT group. This group compensated better after the 30th minute of reperfusion. The control and I/R + DMT groups had similar BFUs after 120 minutes of reperfusion, but in the I/R group BFU was higher. Tubular necrosis developed in the I/R and I/R + DMT groups too; it was moderated under DMT effect, and severe without. Histologic injuries were less in I/R + DMT Group compared to non-treated animals. Histologic changes characteristic to I/R injuries were reversible and microcirculation recovered at the end of 120 minutes reperfusion under the administration of DMT. DMT can be used for renoprotection in kidney transplantation.

Sections du résumé

BACKGROUND BACKGROUND
Ischemia reperfusion (I/R) injury remains one of the most challenging fields of organ transplantation. It is highly associated with the use of expanded criteria donors that might conclude to delayed graft function or early or late graft failure.
OBJECTIVE OBJECTIVE
To investigate the metabolic, microcirculatory parameters, and histologic changes under the effect of N,N-dimethyltryptamine (DMT) in a renal I/R model in rats.
METHOD METHODS
In 26 anesthetized rats both kidneys were exposed. In the control group (n = 6) no other intervention happened. In 20 other animals, the right renal vessels were ligated, and after 60 minutes the right kidney was removed. The left renal vessels were clamped for 60 minutes then released, followed by 120 minutes of reperfusion. In the I/R group (n = 10), there was no additive treatment, while in I/R + DMT group (n = 10) DMT was administered 15 minutes before ischemia. Blood samples were taken, laser Doppler measurement was performed, and both kidneys were evaluated histologically.
RESULTS RESULTS
Microcirculation (blood flux units [BFU]) diminished in all groups, but remarkably so in the I/R + DMT group. This group compensated better after the 30th minute of reperfusion. The control and I/R + DMT groups had similar BFUs after 120 minutes of reperfusion, but in the I/R group BFU was higher. Tubular necrosis developed in the I/R and I/R + DMT groups too; it was moderated under DMT effect, and severe without. Histologic injuries were less in I/R + DMT Group compared to non-treated animals.
CONCLUSION CONCLUSIONS
Histologic changes characteristic to I/R injuries were reversible and microcirculation recovered at the end of 120 minutes reperfusion under the administration of DMT. DMT can be used for renoprotection in kidney transplantation.

Identifiants

DOI: 10.1016/j.transproceed.2019.04.005 PMID: 31101212
pubmed: 31101212
pii: S0041-1345(19)30603-7
doi: 10.1016/j.transproceed.2019.04.005
pii:
doi:

Substances chimiques

Antioxidants 0
Receptors, sigma 0
N,N-Dimethyltryptamine WUB601BHAA

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1268-1275

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Balázs Nemes (B)

Department of Organ Transplantation, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary. Electronic address: nemes.balazs@med.unideb.hu.

Katalin Pető (K)

Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Norbert Németh (N)

Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Anita Mester (A)

Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Zsuzsanna Magyar (Z)

Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Souleiman Ghanem (S)

Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Viktória Sógor (V)

Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Bence Tánczos (B)

Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Ádám Deák (Á)

Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Márk Kállay (M)

Department of Organ Transplantation, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

László Bidiga (L)

Department of Pathology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Ede Frecska (E)

Department of Psychiatry, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › N,N-dimethyltryptamine Prevents Renal...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Agents protecteurs › Antioxydants › N,N-dimethyltryptamine Prevents Renal...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › N,N-dimethyltryptamine Prevents Renal...
questionsmedicales.fr › Appareil urogénital › Voies urinaires › Rein › N,N-dimethyltryptamine Prevents Renal...
questionsmedicales.fr › Procédures de chirurgie opératoire › Procédures de chirurgie urogénitale › Procédures de chirurgie urologique › Transplantation rénale › N,N-dimethyltryptamine Prevents Renal...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Indoles › Tryptamines › N,N-Diméthyl-tryptamine › N,N-dimethyltryptamine Prevents Renal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › N,N-dimethyltryptamine Prevents Renal...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs peptidiques › Récepteur aux neuropeptides › Récepteurs aux opioïdes › Récepteur sigma › N,N-dimethyltryptamine Prevents Renal...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Complications postopératoires › Lésion d'ischémie-reperfusion › N,N-dimethyltryptamine Prevents Renal...