Exocyst Subcomplex Functions in Autophagosome Biogenesis by Regulating Atg9 Trafficking.
Atg9 trafficking
Autophagosome biogenesis
Autophagy
Exocyst
Tethers
Journal
Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R
Informations de publication
Date de publication:
12 07 2019
12 07 2019
Historique:
received:
05
06
2018
revised:
24
04
2019
accepted:
29
04
2019
pubmed:
20
5
2019
medline:
17
6
2020
entrez:
20
5
2019
Statut:
ppublish
Résumé
During autophagy, double-membrane vesicles called autophagosomes capture and degrade the intracellular cargo. The de novo formation of autophagosomes requires several vesicle transport and membrane fusion events which are not completely understood. We studied the involvement of exocyst, an octameric tethering complex, which has a primary function in tethering post-Golgi secretory vesicles to plasma membrane, in autophagy. Our findings indicate that not all subunits of exocyst are involved in selective and general autophagy. We show that in the absence of autophagy specific subunits, autophagy arrest is accompanied by accumulation of incomplete autophagosome-like structures. In these mutants, impaired Atg9 trafficking leads to decreased delivery of membrane to the site of autophagosome biogenesis thereby impeding the elongation and completion of the autophagosomes. The subunits of exocyst, which are dispensable for autophagic function, do not associate with the autophagy specific subcomplex of exocyst.
Identifiants
pubmed: 31103773
pii: S0022-2836(18)30567-9
doi: 10.1016/j.jmb.2019.04.048
pmc: PMC6698439
mid: EMS83963
pii:
doi:
Substances chimiques
ATG9 protein, S cerevisiae
0
Autophagy-Related Proteins
0
Membrane Proteins
0
Multiprotein Complexes
0
Protein Subunits
0
Saccharomyces cerevisiae Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2821-2834Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : DBT-Wellcome Trust India Alliance
ID : 500159-Z-09-Z
Pays : India
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.
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