Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides.
Sorghum
Th1 and Th2 cytokine
modeling
peptide mapping
pollen allergen
profilin
Journal
Medicina (Kaunas, Lithuania)
ISSN: 1648-9144
Titre abrégé: Medicina (Kaunas)
Pays: Switzerland
ID NLM: 9425208
Informations de publication
Date de publication:
21 May 2019
21 May 2019
Historique:
received:
30
01
2019
revised:
16
03
2019
accepted:
17
05
2019
entrez:
24
5
2019
pubmed:
24
5
2019
medline:
20
11
2019
Statut:
epublish
Résumé
Nearly 20-30% of the world's population suffers from allergic rhinitis, among them 15% are progressing to asthma conditions. To map the antigenic determinants responsible for IgE binding, the present study is focused on in silico modeling, simulation of Sorb PF and docking of the Sorb PF peptides (PF1-6) against IgG and IgE, followed by in vivo evaluation of the peptides for its allergenicity in mice. Peptide PF3 and PF4 displayed high docking G-scores (-9.05) against IgE only. The mice sensitized with PF3 peptide showed increased levels of IL5, IL12, TNF-alpha, and GMCSF when compared to other peptides and controls, signifying a strong, Th2-based response. Concurrently, the Th1 pathway was inhibited by low levels of cytokine IL2, IFN-γ, and IL-10 justifying the role of PF3 in allergenic IgE response. Based on the results of overlapping peptides PF3 and PF4, the N-terminal part of the PF3 peptide (TGQALVI) plays a crucial role in allergenic response of Sorghum profilin.
Sections du résumé
BACKGROUND AND OBJECTIVES
OBJECTIVE
Nearly 20-30% of the world's population suffers from allergic rhinitis, among them 15% are progressing to asthma conditions.
MATERIALS AND METHODS
METHODS
To map the antigenic determinants responsible for IgE binding, the present study is focused on in silico modeling, simulation of Sorb PF and docking of the Sorb PF peptides (PF1-6) against IgG and IgE, followed by in vivo evaluation of the peptides for its allergenicity in mice.
RESULTS
RESULTS
Peptide PF3 and PF4 displayed high docking G-scores (-9.05) against IgE only. The mice sensitized with PF3 peptide showed increased levels of IL5, IL12, TNF-alpha, and GMCSF when compared to other peptides and controls, signifying a strong, Th2-based response. Concurrently, the Th1 pathway was inhibited by low levels of cytokine IL2, IFN-γ, and IL-10 justifying the role of PF3 in allergenic IgE response.
CONCLUSIONS
CONCLUSIONS
Based on the results of overlapping peptides PF3 and PF4, the N-terminal part of the PF3 peptide (TGQALVI) plays a crucial role in allergenic response of Sorghum profilin.
Identifiants
pubmed: 31117233
pii: medicina55050178
doi: 10.3390/medicina55050178
pmc: PMC6571906
pii:
doi:
Substances chimiques
Epitopes
0
Profilins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Indian Council of Medical Research
ID : DHR/Plan Scheme/GIA/10/2012
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