iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications.
Animals
Cell Culture Techniques
Cloning, Molecular
/ methods
Gene Editing
/ methods
Genetic Vectors
/ genetics
Integrases
/ genetics
Mice
Mice, Transgenic
Mouse Embryonic Stem Cells
Plasmids
/ genetics
Promoter Regions, Genetic
/ drug effects
Recombination, Genetic
/ drug effects
Tamoxifen
/ pharmacology
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
22 05 2019
22 05 2019
Historique:
received:
01
05
2018
accepted:
23
04
2019
entrez:
24
5
2019
pubmed:
24
5
2019
medline:
25
6
2019
Statut:
epublish
Résumé
Most biomedical research aimed at understanding gene function uses the Cre-Lox system, which consists of the Cre recombinase-dependent deletion of genes containing LoxP sites. This system enables conditional genetic modifications because the expression and activity of the recombinase Cre/CreERT2 can be regulated in space by tissue-specific promoters and in time by the ligand tamoxifen. Since the precise Cre-Lox recombination event is invisible, methods were developed to report Cre activity and are widely used. However, numerous studies have shown that expression of a given Cre activity reporter cannot be assumed to indicate deletion of other LoxP-flanked genes of interest. Here, we report the generation of an inducible dual reporter-Cre mouse allele, iSuRe-Cre. By significantly increasing Cre activity in reporter-expressing cells, iSuRe-Cre provides certainty that these cells have completely recombined floxed alleles. This genetic tool increases the ease, efficiency, and reliability of conditional mutagenesis and gene function analysis.
Identifiants
pubmed: 31118412
doi: 10.1038/s41467-019-10239-4
pii: 10.1038/s41467-019-10239-4
pmc: PMC6531465
doi:
Substances chimiques
Tamoxifen
094ZI81Y45
Cre recombinase
EC 2.7.7.-
Integrases
EC 2.7.7.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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